The self-directing optimization technique was employed to determine the optimal conditions for β-1,3-glucanase production by Trichoderma harzianum in batch culture. A maximum β-1,3-glucanase production of 0.910 U was obtained at a pH (controlled) of 4.9, an aeration rate of 0.9 1/(1)(min), and an agitator speed of 220 rev/min which were found to be the most suitable.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Tangarone, B.; Royer, J.C.; Nakas, J.P.: Purification and characterization of an endo-(1,3)-β-D-glucanase from Trichoderma longibrachiatum. Appl. Environ. Microbiol. 55 (1989) 177–184
Bull, A.T.; Chesters, C.G.C.: The biochemistry of laminarin and the nature of laminarinase. In: Nord, F.F. (Ed.): Advances in enzymology and related subjects of biochemistry, vol. 28, pp. 325–364. New York: Interscience Publishers 1966
Théodore, K.; Panda, T.: Statistical optimization of production medium for β-1,3-glucanase synthesis by Trichoderma harzianum. Biotechnol. Tech. 8 (1994) 381–384
Hendrix, C.: Through the response surface with test tube and pipe wrench. Chemtech 10 (1980) 488–497
Hendrix, C.: What every technologist should know about experimental design. Chemtech 9 (1979) 167–174
Théodore, K.; Panda, T.: Production of β-1,3-glucanase from Trichoderma harzianum in surface and submerged culture processes and its role on protoplast generation from Trichoderma reesei mycelium. Bioproc. Eng. 10 (1994) 161–166
Sharma, A.; Nakas, J.P.: Preliminary characterization of laminarinase from Trichoderma longibrachiatum. Enzyme Microb. Technol. 9 (1987) 89–93
About this article
Cite this article
Théodore, K., Panda, T. Self-directing optimization of β-1,3-glucanase production by Trichoderma harzianum in batch culture. Bioprocess Engineering 14, 111–112 (1996). https://doi.org/10.1007/BF00387966
- Waste Water
- Optimal Condition
- Water Management
- Water Pollution