, Volume 130, Issue 3, pp 323–326

Polyhedral bodies and ribulose 1,5-diphosphate carboxylase of the blue-green alga Anabaena cylindrica

  • G. A. Codd
  • W. D. P. Stewart


The ribulose 1,5-diphosphate carboxylase (RUDP Case E.C. activity of late log phase Anabaena cylindrica Lemm. was measured in vitro in fractions obtained by sucrose density gradient centrifugation. Two peaks of enzymic activity were obtained. One, accounting for about 80% of the total measurable activity occurred at the top of the gradient and appeared to be soluble activity; the second showed maximum activity in the 55% (w/w) sucrose fraction and represented 20% of the total activity. When the distribution of RUDP Case was assayed by immunoprecipitation using antiserum to RUDP Case from Euglena gracilis, the corresponding values were 59% and 41%. Electron microscopy of the various fractions showed that polyhedral bodies, which are sites of RUDP Case activity in other autotrophic prokaryotes, were also most abundant in the 55% (w/w) sucrose fraction.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allen, M.B., Arnon, D.I.: Growth and nitrogen fixation in Anabaena cylindrica Lemm. Plant Physiol. 30, 366–372 (1955).Google Scholar
  2. Codd, G.A., Stewart, W.D.P.: Pathways of glycollate metabolism in the blue-green alga Anabaena cylindrica. Arch. Mikrobiol. 94, 11–28 (1973)PubMedGoogle Scholar
  3. Fay, P.: Metabolic activities of isolated spores of Anabaena cylindrica. J. exp. Bot. 20, 100–109 (1969)Google Scholar
  4. Fogg, G.E., Stewart, W.D.P., Fay, P., Walsby, A.E.: The blue-green Algae. London-New York: Academic Press (1973)Google Scholar
  5. Gerhardt, B., Beevers, H.: Influence of sucrose on protein determination by the Lowry procedure. Analyt. Biochem. 24, 337–339 (1969)Google Scholar
  6. Kannangara, C.G., Van Wyk, D., Menke, W.: Immunological evidence for the presence of latent Ca++-dependent ATPase and carboxydismutase on the thylakoid surface. Z. Naturforsch. 25b, 613–618 (1970)Google Scholar
  7. Kelly, D.P.: Autotrophy: Concepts of lithotrophic bacteria and their organic metabolism. Ann. Rev. Microbiol. 25, 177–210 (1971)Google Scholar
  8. Lord, J.M., Codd, G.A., STewart, W.D.P.: Serolocal comparison of ribulose 1,5-diphosphate carboxylase from Euglena gracilis, Chlorella fusca and several blue-green algae. Pl. Sci. Letter, 4, 377–383 (1975)Google Scholar
  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 264–275 (1951)Google Scholar
  10. Sallal, A.-K.J., Codd, G.A.: The intracellular localization of the glycollate-oxidising enzyme of Anabaena cylindrica. FEBS Letters 56, 230–234 (1975)PubMedGoogle Scholar
  11. Schlegel, H.G.: Mechanisms of chemo-autotrophy. In: Marine ecology, ed. O. Kinne, pp. 9–60. Wiley (1975)Google Scholar
  12. Shively, J.M.: Inclusion bodies of prokaryotes. Ann. Rev. Microbiol. 28, 167–187 (1974)Google Scholar
  13. Shively, J.M., Ball, F., Brown, D.H., Saunders, R.E.: Functional organelles in prokaryotes: Polyhedral bodies (carboxysomes) of Thiobacillus neapolitanus. Science 182, 584–586 (1973)PubMedGoogle Scholar
  14. Shively, J.M., Ball, F.L., Kline, B.W.: Electron microscopy of the carboxysomes (polyhedral bodies) of Thiobacillus neapolitanus. J. Bact. 116, 1405–1411 (1973)PubMedGoogle Scholar
  15. Stewart, W.D.P., Codd, G.A.: Polyhedral bodies (carboxysomes) of nitrogen-fixing blue-green algae. Brit. phycol. J 10, 273–278 (1975)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • G. A. Codd
    • 1
  • W. D. P. Stewart
    • 1
  1. 1.Department of Biological SciencesUniversity of DundeeDundeeUK

Personalised recommendations