Planta

, Volume 151, Issue 2, pp 168–175 | Cite as

Evolution of carboxylating enzymes involved in paramylon synthesis (phosphoenolpyruvate carboxylase and carboxykinase) in heterotrophically grown Euglena gracilis

  • Joël Briand
  • Régis Calvayrac
  • Danielle Laval-Martin
  • Jack Farineau
Article
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Accepted:

Abstract

Heterotrophically grown Euglena synthesize grains of paramylon, its reserve carbohydrate, in a vesicular complex of mitochondrial origin. A CO2 fixation activity in dark grown Euglena was demonstrated in the mitochondria via paramylon. At the beginning of the exponential phase of growth, the activity of phosphoenolpyruvate carboxykinase increases before the augmentation of paramylon.

At the end of the exponential phase, the activity of this enzyme decreases, and low residual levels persist in the transition and stationary phases of growth. The activity of phosphoenolpyruvate carboxylase evolves inversely during the heterotrophic growth of the algae in succinate- or a lactate-containing medium. A compartmentalized scheme of carbon metabolism in mitochondria is presented.

Key words

Carbon metabolism Euglena Mitochondria Paramylon Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase 

Abbreviations

PEP

phosphoenolpyruvate

OAA

oxaloacetate

PGA

phosphoglyceric acid

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Joël Briand
    • 1
  • Régis Calvayrac
    • 1
  • Danielle Laval-Martin
    • 1
  • Jack Farineau
    • 2
  1. 1.Laboratoire des Membranes BiologiquesUniversité Paris VIIParis Cedex 05France
  2. 2.Service de Biophysique de Département de BiologieComissariat à l'Energie AtomiqueGif-sur-YvetteFrance

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