Current Genetics

, Volume 13, Issue 3, pp 261–266 | Cite as

Regeneration of cytoplasmic male sterile protoclones of Nicotiana sylvestris with mitochondrial variations

  • X. Q. Li
  • P. Chetrit
  • C. Mathieu
  • F. Vedel
  • R. De Paepe
  • R. Remy
  • F. Ambard-Bretteville
Original Articles

Summary

One fertile and two male-sterile diploid plants were regenerated from the same callus after two cycles of protoplast culture from fertile Nicotiana sylvestris. Genetic studies indicated that the male-sterile trait was under cytoplasmic control. Progenies of each regenerated plant possessed different mitochondrial (mt) DNA restriction patterns. Both cms protoclone types were characterized by specific mtDNA deletions. In addition, a 40 kD mitochondrially encoded polypeptide is lacking in the cms plants.

Key words

Somaclonal variation Mitochondrial DNA Protein synthesis 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • X. Q. Li
    • 1
  • P. Chetrit
    • 1
  • C. Mathieu
    • 1
  • F. Vedel
    • 1
  • R. De Paepe
    • 2
  • R. Remy
    • 1
  • F. Ambard-Bretteville
    • 1
  1. 1.Génétique Moléculaire des Plantes, UA 115Université Paris-SudOrsayFrance
  2. 2.Génétique et Physiologie du Développement des PlantesCNRSGif/YvetteFrance
  3. 3.Department of BiologyPeking UniversityBeijingChina

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