Expression of the oxi1 and maturase-related RF1 genes in yeast mitochondria
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Transcription of the yeast mitochondrial oxi1 gene (cytochrome oxidase subunit 2) is initiated at a variant non a nucleotide sequence, TTAAAAGTA, located 54 by upstream from the protein-coding gene. Transcriptional initiation at this site gives rise to a 2,500 nucleotide primary transcript containing both the oxi1 gene and the downstream maturase-related reading frame, RF1. Precise transcript mapping has revealed that the 3′-end of the mature oxi1 mRNA is generated by an endonucleolytic cleavage which takes place after the conserved dodecamer sequence, AAUAAUAUUCUU (End-of-Messenger signal), 75 nucleotides downstream from the oxi1 stop codon. Since the RF1 5′-terminal coding region overlaps the oxi1 3′-terminal coding sequence, cleavage at this motif truncates the RF1 message suggesting that the expression of the putative RF1 protein is controlled at the level of dodecamer processing.
Key wordsSaccharomyces cerevisiae mitochondria oxi1 gene Maturase-related ORF Transcription initiation End-of-Messenger signal
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