Red light treatment in vitro increases the pelletability of phytochrome in homogenates of etiolated barley (Hordeum vulgare L. cv. ‘Julia’) leaves. When mixtures of soluble phytochrome (100,000 x g supernatant) and partially-purified organelles (Sephadex G-50 eluate) are irradiated the amount of pelletable phytochrome increases by a factor of two. Pre-irradiation treatments show that phytochrome in both components of the mixture must be in the Pfr form for increased pelletability to be observed. Once associated, photoreversion of Pfr to Pr does not result in decreased pelletability. The results are consistent with a non-artifactual in vitro association of soluble phytochrome to organelle membranes. One possible explanation is that Pfr molecules associate to form dimers.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Cooke, R.J., Saunders, P.F.: Phytochrome-mediated changes in extractable gibberellin activity in a cell-free system isolated from etiolated wheat leaves. Planta 124, 319–328 (1975)
Evans, A., Smith, H.: Localization of phytochrome in etioplasts and its regulation in vitro of gibberellin levels. Proc. Natl. Acad. Sci. USA 73, 138–42 (1976)
Furuya, M., Manabe, K.: Phytochrome in mitochondrial and microsomal fractions isolated from etiolated pea shoots. In: Light and plant development pp. 143–155, Smith, H., ed. London: Butterworths 1976
Georgevitch, G., Cedel, T.E., Roux, S.J.: Use of 125I-labelled phytochrome to quantitate phytochrome binding to membranes of Avena sativa, Proc. Natl. Acad. Sci. USA 74, 4439–43 (1977)
Hendricks, S.B., Borthwick, H.A.: The function of phytochrome in regulation of plant growth. Proc. Natl. Acad. Sci. USA 58, 2125–30 (1967)
Lowry, O.H., Rosenborough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–273 (1951).
Manabe, K., Furuya, H.: Phytochrome-dependent reduction of nicotinamide nucleotides in the mitochondrial fraction isolated from etiolated pea epicotyls. Plant Physiol. 53, 343–347 (1974)
Marmé, D.: Phytochrome: Membranes as possible sites of primary action. Ann. Rev. Plant Physiol. 28, 173–98 (1977)
Pratt, L.H.: Phytochrome properties in vitro. Photochem. Photobiol. (in press) (1977)
Pratt, L.H., Marmé, D.: Red-light enhanced phytochrome pelletability: a re-examination and further characterization. Plant Physiol. 58, 686–92
Quail, P.H.: Particle-bound phytochrome: association with a ribonucleoprotein fraction from Cucurbita pepo L. Planta 123, 223–34 (1975)
Quail, P.H.: How “pure” are G-50 plastids? Proc. Annual European Symposium on Photomorphogenesis, Bet Dagan, Israel p. 78 1977
Quail, P.H., Gressel, J.: Particle-bound phytochrome: Interaction of the pigment with ribonucleoprotein material from cucubita pepo L. In: Light and plant development pp. 111–128, Smith, H., ed. London: Butterworths 1976
Rubinstein, B.K., Drury, K.S., Park, R.B.: Evidence for bound phytochrome in oat seedlings. Plant Physiol. 44, 105–109 (1969).
Schäfer, E.: Analysis of the binding of phytochrome to particulate fractions. Photochem. Photobiol. 21, 189–91 (1975)
Steinitz, B.H., Drumm, H., Mohr, H.: The appearance of competence for phytochrome-mediated anthocyanin synthesis in the cotyledons of Sinapis alba L. Planta 130, 23–31 (1976)
Wellburn, A.R., Wellburn, F.A.M.: A new method for the isolation of eitoplasts with intact envelopes. J. Exp. Bot. 23, 972–979 (1971)
About this article
Cite this article
Smith, H., Evans, A. & Hilton, J.R. An in vitro association of soluble phytochrome with a partially purified organelle fraction from barley leaves. Planta 141, 71–76 (1978). https://doi.org/10.1007/BF00387747