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Planta

, Volume 107, Issue 2, pp 161–170 | Cite as

Development and differentiation of haploid Lycopersicon esculentum (tomato)

  • Peter M. Gresshoff
  • Colin H. Doy
Article

Summary

Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture. This is a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana. Differentiation of haploid callus of Lycopersicon esculentum can be controlled both in the dark and the light by hormones added to defined minimal media. Development to plantlets is achieved only in the light. Callus cells can be induced to develop into seedless pseudo-fruits. Chromosome counts on callus cells or root-tip cells establishes haploidy (n=12).

Haploidy can be maintained in culture on defined minimal media for at least one year.

Keywords

Arabidopsis Thaliana Minimal Medium Anther Culture Lycopersicon Esculentum Chromosome Count 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Blaydes, D. F.: Interaction of kinetic and various inhibitors in the growth of soy bean tissue. Physiol. Plantar. 19, 748–753 (1966).Google Scholar
  2. Darlington, C. D., LaCour, L. F.: Handling of chromosomes, 2nd edn., revised. London: George Allen and Unwin 1947.Google Scholar
  3. Doy, C. H., Gresshoff, P. M., Rolfe, B.: Phage-mediated transfer and subsequent expression of the galactose operon from Escherichia coli in cells of haploid tomato callus. Proc. Austr. Biochem. Soc. 5 (1972a).Google Scholar
  4. Doy, C. H., Gresshoff, P. M., Rolfe, B.: Transgenosis of bacterial genes from Escherichia coli to cultured cells of haploid Lycopersicon esculentum. Proc. Int. Meeting “The biochemistry of gene expression in higher organisms”. (1972b).Google Scholar
  5. Gamborg, O. L., Eveleigh, D.: Culture methods and detection of glucanases in suspension cultures of wheat and barley. Canad. J. Biochem. 46, 417–421 (1968).Google Scholar
  6. Gresshoff, P. M., Doy, C. H.: Haploid Arabidopsis thaliana callus and plants from anther culture. Aust. J. biol. Sci. 25, 259–264 (1972).Google Scholar
  7. Guha, S., Maheshwari, S. C.: Cell division and differentiation of embryos in pollen grains of Datura in vitro. Nature (Lond.) 212, 97–98 (1966).Google Scholar
  8. Nataka, K., Tanaka, M.: Differentiation of embryoids from developing germ cells in anther culture of tobacco. Jap. J. Genet. 43, 65–71 (1968).Google Scholar
  9. Sunderland, N., Wicks, F. M.: Cultivation of haploid plants from tobacco pollen. Nature (Lond.) 244, 1227–1229 (1969).Google Scholar
  10. Sunderland, N., Wicks, F. M.: Embryoid formation in pollen grains of Nicotiana tabacum. J. exp. Bot. 22, 213–221 (1971).Google Scholar
  11. Sunderland, N.: Anther culture: a progress report. Sci. Progr. Oxf. 59, 527–549 (1971).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Peter M. Gresshoff
    • 1
  • Colin H. Doy
    • 1
  1. 1.Department of Genetics, Research School of Biological SciencesAustralian National UniversityCanberra CityAustralia

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