, Volume 107, Issue 2, pp 161–170 | Cite as

Development and differentiation of haploid Lycopersicon esculentum (tomato)

  • Peter M. Gresshoff
  • Colin H. Doy


Haploid callus cultures of selected races of Lycopersicon (tomato) species can be obtained from anther culture. This is a further demonstration of a proposed general method of haploid culture developed with Arabidopsis thaliana. Differentiation of haploid callus of Lycopersicon esculentum can be controlled both in the dark and the light by hormones added to defined minimal media. Development to plantlets is achieved only in the light. Callus cells can be induced to develop into seedless pseudo-fruits. Chromosome counts on callus cells or root-tip cells establishes haploidy (n=12).

Haploidy can be maintained in culture on defined minimal media for at least one year.


Arabidopsis Thaliana Minimal Medium Anther Culture Lycopersicon Esculentum Chromosome Count 
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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Peter M. Gresshoff
    • 1
  • Colin H. Doy
    • 1
  1. 1.Department of Genetics, Research School of Biological SciencesAustralian National UniversityCanberra CityAustralia

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