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Polyphenol synthesis in cell suspension cultures of Paul's Scarlet rose

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Summary

  1. 1.

    In dark-grown cell suspension cultures of Paul's Scarlet rose (Rosa sp.), polyphenol accumulation is essentially restricted to late- and post-exponential phase cells. The range of polyphenols synthesised is extensive, involving at least fourteen different compounds which show a characteristic pattern of development over the growth cycle.

  2. 2.

    The duration of polyphenol accumulation is largely determined by the availability of carbohydrate, while the initiation and initial rate of synthesis is largely influenced by a complex of factors of which auxin concentration and light are most important. High 2,4-D concentrations (5x10-5M) supress accumulation by delaying initiation and reducing the subsequent rate of synthesis. High intensity illumination (6000 Lux) partially reverses the inhibition of polyphenol synthesis at 5x10-5M 2,4-D, and enhances accumulation at lower auxin concentrations (5x10-7M 2,4-D).

  3. 3.

    Illuminated cultures also accumulate anthocyanin. Initiation of pigment synthesis is insensitive to the auxin concentration of the medium, and higher hormonal levels (5x10-5M 2,4-D) increase net anthocyanin accumulation by prolonging the period over which it is synthesised.

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References

  1. Berlin, J., Barz, W.: Stoffwechsel von Isoflavonen und Cumostanen in Zell- und Callussuspensionskulturen von Phaseolus aureus Roxb. Planta (Berl.) 98, 300–314 (1971).

  2. Blakely, L. M., Steward, F. C.: Growth induction in cultures of Haplopappus gracilis. I. The behaviour of cultured cells. Amer. J. Both. 48, 351–358 (1961).

  3. Cartwright, R. A., Roberts, E. A. H.: Theogallin, a polyphenol occurring in tea. J. Sci. Fd. Agric. 5, 593–597 (1954).

  4. Constabel F.: Phenolics in tissue cultures derived from Juniperus communis L. Studies on tannin synthesis. Proc. Int. Conf. Plant Tissue Cultures. Penn. State University, p. 183 (1963).

  5. Constabel, F.: Gerbstoffproduktion der Calluskulturen von Juniperus communis L. Planta (Berl.) 79, 58–64 (1968).

  6. Constabel, F., Shyluk, J. P., Gamborg, O. L.: The effect of hormones on anthocyanin accumulation in cell cultures of Haplopappus gracilis. Planta (Berl.) 96, 306–316 (1971).

  7. Davies, M. E.: Multisample exyme extraction from cultured plant cell suspensions. Plant Physiol. 47, 38–42 (1971).

  8. Davies, M. E.: Effects of auxin on polyphenol accumulation and the development of phenylalanine ammonia-lyase activity in darkgrown suspension cultures of Paul's Scarlet rose. Planta (Berl.) 104, 66–77 (1972).

  9. Forrest, G. I.: Studies on the polyphenol metabolism of tissue cultures derived from the tea plant (Camellia sinensis L.) Biochem. J. 113, 765–772 (1969).

  10. Forrest, G. I., Bendall, D. S.: The distribution of polyphenols in the tea plant (Camellia sinensis L). Biochem. J. 113, 741–755 (1969).

  11. Hahlbrock, K., Wellmann, E.: Light-induced flavone biosynthesis and activity of phenylalanine ammonia-lyase and UDP-apiose synthetase in cell suspension cultures of Petroselinum hortense. Planta (Berl.) 94, 236–239 (1970).

  12. Kaul, B., Staba, E. J.: Ammi visnaga (L.) Lam. tissue cultures. Multiliter suspension growth and examination for furanochromes. Planta med. (Stuttg.) 15, 145–156 (1967).

  13. Miller, C.O.: Control of deoxyisoflavone synthesis in soybean tissue. Planta (Berl.) 87, 26–35 (1969).

  14. Nash, D. T., Davies, M. E.: Some aspects of growth and metabolism of Paul's Scarlet rose cell suspensions. J. exp. Bot. (in press).

  15. Reinhard, E.: Probleme der Produktion von Arzneistoffen durch pflanzliche Gewebekulturen. Dtsch. Apoth.-Ztg 107, 1201–1207 (1967).

  16. Reinhard, E., Corduan, G., Volk, O. H.: Über Gewebekulturen von Ruta graveolens. Planta med. (Stuttg.) 16, 8–16 (1968).

  17. Roberts, E. A. H., Cartwright, R. A., Wood, D. J.: The flavonols of tea. J. Sci. Fd. Agric. 7, 637–646 (1956).

  18. Roux, D. G.: Some recent advances in the identification of leuco-anthocyanins and the chemistry of condensed tannins. Nature (Lond.) 180, 973–975 (1957).

  19. Siegelman, H. W.: Physiological studies on phenolic biosynthesis. In: Biochemistry of phenolic compounds, p. 437–465 (J. B. Harborne, ed.). New York: Academic Press 1964.

  20. Staba, E. J.: Plant tissue cultures as a technique for the phytochemist. Recent Adv. Phytochem. 2, 75–106 (1969).

  21. Strauss, J.: Anthocyanin synthesis in corn endosperm tissue cultures. II. Effect of certain inhibitor and stimulatory agents. Plant Physiol. 35, 645–650 (1960).

  22. Sugano, N., Hayashi, K.: Detection of aromatic acid ammonia-lyases in carrot aggregens in relation to the biosynthesis of anthocyanin and related phenolic compounds. Studies on anthocyanins, LX. Bot Mag. (Tokyo) 81, 371–376 (1968).

  23. Swain, T., Hillis, W. E.: The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J. Sci. Fd. Agric. 10, 63–68 (1959).

  24. Szweykowska, A.: The effect of nitrogen feeding on anthocyanin synthesis in isolated red cabbage embryos. Acta Soc. Bot. Polon. 28, 539–549 (1959).

  25. Tabata, M., Yamamoto, H., Hiraoka, N., Marumoto, Y., Konoshima, M.: Regulation of nicotine production in tobacco tissue culture by plant growth regulators. Phytochem. 10, 723–729.

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Davies, M.E. Polyphenol synthesis in cell suspension cultures of Paul's Scarlet rose. Planta 104, 50–65 (1972). https://doi.org/10.1007/BF00387683

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Keywords

  • Polyphenol
  • Cell Suspension Culture
  • Characteristic Pattern
  • Phase Cell
  • Growth Cycle