Kinetic studies of anthocyanin formation in far-red and red light given in various combinations have shown that the responses to the two wave-lengths are different and depend on the sequence of irradiation. Red light reduced the effectiveness of subsequent prolonged far-red regardless of whether red began the irradiation or was interposed after several hours of far-red. However, far-red eventually regained some activity to a degree which depended on seedling age and the duration of the initial exposure to red light. It is suggested that red light may act by destroying the labile form of phytochrome (presumed to be required for far-red action) and that the resumption of far-red activity may indicate new synthesis of such phytochrome.
When given after several hours of far-red, the yield in prolonged red light was markedly increased and, in intact hypocotyls, anthocyanin synthesis then proceeded as rapidly in red as in far-red. Far-red also increased the yield in subsequent red light when given after an initial irradiation with read and, under these conditions, anthocyanin yield in hypocotyls was even greater if seedlings were transferred to red light than if the far-red was continued. Red light appeared to favour formation of anthocyanin in the hypocotyl and it is possible that the action of red is to block synthesis in the cotyledons thus leading to an increased translocation of substrate to the hypocotyl. A number of treatments increased the yield of cotyledons in red light but, unlike the hypocotyls, they never reached that obtained with far-red. Several observations indicated that the increased effectiveness of red, when given after far-red, might be due to the accumulation of substrate in far-red, which is then converted to anthocyanin by the action of the stable form of phytochrome.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Butler, W. L., and H. W. Siegelman: Nonphotochemical transformations of phytochrome in vivo. Plant Physiol. 38, 514–519 (1963).
Clarkson, D. T., and W. S. Hillman: Apparent phytochrome synthesis in Pisum tissue. Nature (Lond.) 213, 468–470 (1967a).
——: Stability of phytochrome concentration in dicotyledonous tissues under continuous far-red light. Planta (Berl.) 75, 286–290 (1967b).
Evans, L. T., S. B. Hendricks, and H. A. Borthwick: The role of light in suppressing hypocotyl elongation in lettuce and Petunia. Planta (Berl.) 64, 201–218 (1965).
Grill, R.: Photocontrol of anthocyanin formation in turnip seedlings. I. Demonstration of phytochrome action. Planta (Berl.) 66, 293–300 (1965).
—: Photocontrol of anthocyanin synthesis in turnip seedlings. IV. The effect of feeding precursors. Planta (Berl.) 76, 11–24 (1967).
Grill, R.: Phytochrome changes in turnip seedlings in darkness, far-red light or following exposure to red irradiation. 5th Internat. Congr. on Photobiology (Hanover) 64 (1968).
—, and D. Vince: Anthocyanin formation in turnip seedlings (Brassica rapa L.): Evidence for two light steps in the biosynthetic pathway. Planta (Berl.) 63, 1–12 (1964).
——: Photocontrol of anthocyanin formation in turnip seedlings. II. The possible role of phytochrome in the response to prolonged irradiation with far-red or blue light. Planta (Berl.) 67, 122–135 (1965).
——: Photocontrol of anthocyanin formation in turnip seedlings. III. The photoreceptors involved in the responses to prolonged irradiations. Planta (Berl.) 70, 1–12 (1966).
Hartmann, K. M.: A general hypothesis to interpret ‘high energy phenomena’ of photomorphogenesis on the basis of phytochrome. Photochem. Photobiol. 5, 349–366 (1966).
Hartmann, K. M.: Identification of the photoreceptor of the ‘high energy photomorphogenetic reaction’ for the far-red region. 5th Internat. Congr. on Photobiology (Hanover) 10 (1968).
Lane, H. C., and M. J. Kasperbauer: Photomorphogenic responses of dodder seedlings. Plant Physiol. 40, 109–116 (1965).
Lange, H., I. Bienger u. H. Mohr: Eine neue Beweisführung für die Hypothese einer differentiellen Genaktivierung durch Phytochrom 730. Planta (Berl.) 76, 359–366 (1967).
Lint, P. J. A. L. de, and C. J. P. Spruit: Phytochrome destruction following illumination of mesocotyls of Zea Mays L. Meded. Landbouwhogeschool. Wageningen 63, 1–7 (1963).
Mohr, H., E. Wagner u. K. M. Hartmann: Zur Deutung der Hochenergiereaktion der Photomorphogenese (=HER) auf der Basis des Phytochrom-systems. Naturwissenschaften 52, 209 (1965).
Rollin, P., and G. Maignan: Phytochrome and the photoinhibition of germination. Nature (Lond.) 214, 741–742 (1967).
Vince, D., and R. Grill: The photoreceptors involved in anthocyanin synthesis. Photochem. Photobiol. 5, 407–411 (1966).
Wagner, E., u. H. Mohr: Kinetische Studien zur Interpretation der Wirkung von Sukzedanbestrahlungen mit Hellrot und Dunkelrot bei der Photomorphogenese (Anthocyansynthese bei Sinapis alba L.). Planta (Berl.) 70, 34–41 (1966).
About this article
Cite this article
Grill, R. Photocontrol of anthocyanin formation in turnip seedlings. Planta 85, 42–56 (1969). https://doi.org/10.1007/BF00387660
- Kinetic Study
- Stable Form
- Initial Exposure
- Anthocyanin Synthesis
- Initial Irradiation