Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Ribosomal and mitochondrial peroxidase isoenzymes of the lentil (Lens culinaris) root

  • 23 Accesses

  • 11 Citations


Ribosome- and mitochondria-rich preparations were obtained from lentil roots and their peroxidase isoenzymes examined by starch gel electrophoresis. Seven isoenzymes were shown to be associated with both the ribosomal and mitochondrial fractions.

The apparent similarity between ribosomal and mitochondrial isoenzyme patterns, together with the observation that a considerable amount of peroxidase activity dissociated from the ribosomes during sedimentation of the ribosomal preparation into a sucrose gradient, suggested that at least some of these isoperoxidases were attached to membranes.

This is a preview of subscription content, log in to check access.


  1. Baxter, R., Hanson, J. B.: The effect of 2,4-dichlorophenoxyacetic acid upon the metabolism and composition of soybean hypocotyl mitochondria. Planta (Berl.) 82, 246–260 (1968).

  2. Darimont, E., Gaspar, Th.: A propos du nombre et du poids moléculaire des isoenzymes peroxydasiques de la racine de Lens culinaris. In: Aspects moléculaires du développement. Bull. Soc. Bot. France. In press (1971).

  3. Gaspar, Th.: Effet comparé de l'Amo-1618 et de l'AIA sur les peroxydases ribosomiques et cytoplasmiques de la racine de Lentille. Physiol. Vég. (Paris) 8, 641–648 (1970).

  4. Hackett, D. P., Ragland, F. E.: Oxidation of menadiol by fractions isolated from non-photosynthetic plant tissues. Plant. Physiol. 37, 656–662 (1962).

  5. Ivanova, T. H., Davydova, M. A., Rubin, B. A.: Mitochondrial peroxidase and its probable role in oxidative processes. Biokhimiya 31, 1167–1173 (1966).

  6. Jermyn, M. A., Thomas, R.: Multiple components in horseradish peroxidase. Biochem. J. 56, 631–639 (1954).

  7. Lanzani, G. A., Galante, E.: Peroxidase activities from wheat embryo ribosomes. Arch. Biochim. Biophys. 106, 20–24 (1964).

  8. Lin, C. Y., Key, J. L., Bracker, C. E.: Association of D-RNA with polyribosomes in the soybean root. Plant Physiol. 41, 976–982 (1966).

  9. Penon, P., Cecchini, J. P., Miassod, R., Ricard, J., Teissere, M., Pinna, M. H.: Peroxidases associated with lentil root ribosomes. Phytochem. 9, 73–86 (1970).

  10. Ridge, I., Osborne, D. J.: Hydroxyproline and peroxidases in cell walls of Pisum sativum: regulation by ethylene. J. exp. Bot. 21, 843–856 (1970).

  11. Sagi, F.: Subcellular localization of indole-3-acetic acid oxidase in the hypocotyl cells of Lupinus albus L. Botanikai Közlemenyek 57, 251–257 (1970).

  12. Schwachhofer, K., Gaspar, Th.: Isoperoxydases et hydroxyproline dans les parois cellulaires des racines de Lentille. Biochim. biophys. Acta (Amst.). In press (1973).

  13. Scopes, R. K.: Methods for starch gel electrophoresis of sarcoplasmic proteins. Biochem. J. 107, 139–150 (1968).

  14. Shannon, L. M.: Plant isoenzymes. Ann. Rev. Plant. Physiol. 19, 187–201 (1968).

  15. Siegel, B. Z., Galston, A. W.: The isoperoxidases of Pisum sativum. Plant Physiol. 42, 221–226 (1967).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Darimont, E., Baxter, R. Ribosomal and mitochondrial peroxidase isoenzymes of the lentil (Lens culinaris) root. Planta 110, 205–212 (1973). https://doi.org/10.1007/BF00387633

Download citation


  • Sucrose
  • Starch
  • Sedimentation
  • Electrophoresis
  • Peroxidase Activity