Planta

, Volume 101, Issue 2, pp 166–170

Effects of temperature on accumulation of starch or lipid in chloroplasts of grapevine

  • M. S. Buttrose
  • C. R. Hale
Short Communication

Summary

The amount of starch accumulated in chloroplasts of grapevine leaves fell, with increasing temperature, from 23% of dry weight at 18/13° C (day/night) to 1% at 35/30° C, whereas total lipids in the leaf rose from 6 to 16% of dry weight. Preparations viewed by electron microscopy had large starch granules in the chloroplasts at 18/13° C, and large lipid droplets at 35/30° C. At 35/30° C chlorophyll content of chloroplasts was high and grana prominent, suggesting that the results were due to selective effects of temperature on metabolic pathways.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Addoms R.MM., Mounce, F. C.: Further notes on the nutrient requirements and the histology of the cranberry, with special reference to the sources of nitrogen. Plant Physiol. 7, 643–656 (1932).Google Scholar
  2. Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).Google Scholar
  3. Ballantine, J. E. M., Forde, B. J.: The effect of light intensity and temperature on plant growth and chloroplast ultrastructure in soybean. Amer. J. Bot. 57, 1150–1159 (1970).Google Scholar
  4. Bligh, E. G., Dyer, W. J.: A rapid method for total lipid extraction and purification. Canad. J. Biochem. Physiol. 37, 911–917 (1959).Google Scholar
  5. Butt, V. S., Beevers, H.: The plant lipids. In: Plant physiology—a treatise, vol. 4 B, pp. 265–414 (F. C.Steward, ed.). New York: Acad. Press 1966.Google Scholar
  6. Buttrose, M. S.: Use of carbohydrate reserves during growth from cuttings of grape vine. Aust. J. biol. Sci. 19, 247–256 (1966).Google Scholar
  7. Dodge, J. D.: Changes in chloroplast fine structure during the autumnal senescence of Betula leaves. Ann. Bot. 34, 817–824 (1970).Google Scholar
  8. Klein, S.: The effect of low temperature on the development of the lamellar system in chloroplasts. J. biophys. biochem. Cytol. 8, 529–538 (1960).PubMedGoogle Scholar
  9. Laetsch, W. M.: Chloroplast specialization in dicotyledons possessing the C4-dicarboxylic acid pathway of photosynthetic CO2 fixation. Amer. J. Bot. 55, 875–883 (1968).Google Scholar
  10. Martin, J. T.: Determination of the components of plant cuticles. J. Sci. Food Agric. 11, 635–640 (1960).Google Scholar
  11. McWilliam, J. R., Naylor, A. W.: Temperature and plant adaptation. I. Interaction of temperature and light in the synthesis of chlorophyll in corn. Plant Physiol. 42, 1711–1715 (1967).Google Scholar
  12. Spurr, A. R.: A low-viscosity expoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969).PubMedGoogle Scholar
  13. Taylor, A. O., Craig, A. S.: Plants under climatic stress. II. Low temperature, high light effects on chloroplast ultrastructure. Plant Physiol. 47, 719–725 (1971).Google Scholar
  14. — Rowley, J. A.: Plants under climatic stress. I. Low temperature, high light effects on photosynthesis. Plant Physiol. 47, 713–718 (1971).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • M. S. Buttrose
    • 1
  • C. R. Hale
    • 1
  1. 1.Division of Horticultural ResearchCSIROAdelaide

Personalised recommendations