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Carbohydrate utilisation by microbial symbionts in the marine herbivorous fishes Odax cyanomelas and Crinodus lophodon

Abstract

Carbohydrate uptake and catabolism by the gut microbiota of two species of temperate marine herbivorous fish were investigated using enzyme extracts prepared from microbial pellets. The fish studied were the herring cale Odax cyanomelas (Family Odacidae), which feeds on Ecklonia radiata, and the sea carp Crinodus lophodon (Family Aplodactylidae), which feeds primarily on red and green algae. Constitutive phosphoenolpyruvate phosphotransferase systems for glucose, galactose, fructose and mannitol were present in the microbiota of both fish. Hexokinase, fructokinase and mannitol dehydrogenase activities indicated that transport of the corresponding substrates may be coupled to permeases. Galactokinase activity was only detected in C. lophodon, as expected from its diet. Phosphofructokinase and pyruvate kinase activities were taken to indicate that carbohydrate metabolism proceeded via the fructose bisphosphate pathway. Differences in the transport and metabolism of the different monomers by the microbiota of O. cyanomelas and C. lophodon correlated strongly with predicted monomer availability in the gut of each species, suggesting that the microbiota are an integral component of digestion in these fish. The rates of production in adult fish of acetate, the major short-chain fatty acid, were estimated as 136 μmol·h-1 in O. cyanomelas and 166 μmol·h-1 in C. lophodon. These rates indicate that microbial fermentation is a potentially important source of energy for the host fish.

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Abbreviations

AK :

acetate kinase

CTAB :

cetyl trimethylammonium bromide

FK :

fructokinase

F1P:

fructose 1-phosphate

F1PK :

fructose 1-phosphate kinase

F1-6BP :

Fructose 1,6-bisphosphate

F6P :

fructose 6-phosphate

GK :

galactokinase

Gal1P :

galactose 1-phosphate

G6P :

glucose 6-phosphate

HK :

hexokinase

MDH :

mannitol dehydrogenase

M1P :

mannitol 1-phosphate

M1-PDH :

mannitol 1-phosphate dehydrogenase

PEP :

phosphoenolpyruvate

PFK :

phosphofructokinase

PK :

pyruvate kinase

PTS :

phosphotransferase system

SCFA :

short-chain fatty acid(s)

TFA :

trifluoroacetic acid

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Author information

Correspondence to M. Slaytor.

Additional information

Communicated by I. D. Hume

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Seeto, G.S., Veivers, P.C., Clements, K.D. et al. Carbohydrate utilisation by microbial symbionts in the marine herbivorous fishes Odax cyanomelas and Crinodus lophodon . J Comp Physiol B 165, 571–579 (1996). https://doi.org/10.1007/BF00387519

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Key words

  • Temperate marine fish
  • Marine herbivorous fish
  • Carbohydrate fermentation
  • Herring cale, Odax cyanomelas
  • Sea carp, Crinodus lophodon