Physiological aspects of conjugation in fission yeast
- 115 Downloads
Conjugation was studied in Schizosaccharomyces pombe using liquid media. Nitrogen, which was growth-limiting in a synthetic medium, had to be consumed completely before conjugation could start. Conjugation was preceded by sexual agglutination. Agglutinability was not constitutive in heterothallic strains. It only developed when cells of h+ and h- mating type were grown in mixed culture for at least 2.5 hr before the start of conjugation.
Unable to display preview. Download preview PDF.
- Bresch, C., Müller, G., Egel, R.: Genes involved in meiosis and sporulation of a yeast. Molec. Gen. Genetics 102, 301–306 (1968).Google Scholar
- Crandall, M. A., Brock, T. D.: Molecular basis of mating in the yeast Hansenula wingei. Bact. Rev. 32, 139–163 (1968).Google Scholar
- Croes, A. F.: Induction of meiosis in yeast. II. Metabolic factors leading to meiosis. Planta (Berl.) 76, 227–237 (1967).Google Scholar
- Duntze, W., MacKay, V., Manney, T. R.: Saccharomyces cerevisiae: A diffusible sex factor. Science 168, 1472–1473 (1970).Google Scholar
- Johnson, B. F.: Growth of the fission yeast Schizosaccharomyces pombe with late, eccentric, lytic fission in an unbalanced medium. J. Bact. 94, 192–195 (1967).Google Scholar
- Leupold, U.: Genetical methods for Schizosaccharomyces pombe. In: Methods in cell physiology, vol. 4, p. 169–177 (D. M. Prescott, ed.). New York: Acad. Press 1970.Google Scholar
- Levi, J. D.: Mating reaction in yeast. Nature (Lond.) 177, 753–754 (1956).Google Scholar
- Mitchison, J. M.: Physiological and cytological methods for Schizosaccharomyces pombe. In: Methods in cell physiology, vol. 4, p. 131–168 (D. M. Prescott, ed.). New York: Acad. Press 1970.Google Scholar
- Plempel, M.: Die chemischen Grundlagen der Sexualreaktion bei Zygomyceten. Planta (Berl.) 59, 492–508 (1963).Google Scholar
- Raper, J. R.: Sexual hormones in Achlya. Amer. Sci. 39, 110–121 (1951).Google Scholar
- Rosen, H.: A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. 67, 10–15 (1957).Google Scholar