Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Gibberellic acid and the fine structure of barley aleurone cells

I. Changes during the lag-phase of α-amylase synthesis

  • 67 Accesses

  • 75 Citations


This paper describes changes in the fine structure of barley aleurone cells following treatment with gibberellic acid (GA3). Within 2 hr of GA3 treatment the aleurone grains lose the spherical appearance characteristic of aleurone cells incubated in water and buffer alone. This swelling increases with increased exposure of the cells to GA3 and reaches a maximum at about 10 hr. Accompanying this increase in volume of the aleurone grains is an increase in the amount of rough endoplasmic reticulum. The relevance of these GA3-stimulated changes in aleurone-cell fine-structure to GA3-regulated α-amylase production is discussed.

This is a preview of subscription content, log in to check access.


  1. Chrispeels, M. J., and J. E. Varner: Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406 (1967a).

  2. ——: Hormonal control of enzyme synthesis: on the mode of action of gibberellic acid and abscisin in aleurone layers of barley. Plant Physiol. 42, 1008–1016 (1967b).

  3. Eb, A. A. Van der, and P. J. Nieuwdorp: Electron microscopic structure of the aleurone cells of barley during germination. Acta bot. neerl. 15, 690–699 (1967).

  4. Filner, P., and J. E. Varner: A simple and unequivocal test for de novo synthesis of enzymes: density labeling of barley α-amylase with H2O18. Proc. nat. Acad. Sci. (Wash.) 58, 1520–1526 (1967).

  5. Jacobsen, J. V., and J. E. Varner: Gibberellic acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 42, 1596–1600 (1967).

  6. Jones, R. L.: The fine structure of barley aleurone cells. Planta (Berl.) 85, 359–375 (1969).

  7. —, and J. E. Varner: The bioassay of gibberellins. Planta (Berl.) 72, 53–59 (1967).

  8. Paleg, L.: Physiological effects of gibberellic acid. II. On starch hydrolyzing enzymes of barley endosperm. Plant Physiol. 35, 902–906 (1960).

  9. —, and B. Hyde: Physiological effects of gibberellic acid. VII. Electron microscopy of barley aleurone cells. Plant Physiol. 39, 673–680 (1964).

  10. Varner, J. E.: Gibberellic acid controlled synthesis of α-amylase in barley endosperm. Plant Physiol. 39, 413–415 (1964).

  11. —, and G. Ram Chandra: Hormonal control of enzyme synthesis in barley endosperm. Proc. nat. Acad. Sci. (Wash.) 52, 100–106 (1964).

  12. Yomo, H.: Studies on the α-amylase activating substance. IV. On the amylase activating action of gibberellin. Hakko Kyokaishi 18, 600–602 (1960).

Download references

Author information

Additional information

Work supported by National Science Foundation grants GB-5863 and GB-8332. The skillful technical assistance of Mrs. Janet Price is gratefully acknowledged.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Jones, R.L. Gibberellic acid and the fine structure of barley aleurone cells. Planta 87, 119–133 (1969). https://doi.org/10.1007/BF00386971

Download citation


  • Endoplasmic Reticulum
  • Fine Structure
  • Gibberellic Acid
  • Rough Endoplasmic Reticulum
  • Appearance Characteristic