Planta

, Volume 102, Issue 3, pp 190–205 | Cite as

Soluble electron-transport activities in fresh and aged turnip tissue

  • J. M. Rungie
  • J. T. Wiskich
Article
Received:

Summary

A soluble (supernatant) fraction from turnips catalyses the reduction of both FeCN and DCPIP but usually not cytochrome c in the presence of either NADH or NADPH. Slicing and aging turnip tissue induces an increase in these activities as well as the development of an NADH-cytochrome c reductase activity.

(NH4)2SO4 and Sephadex fractionation indicated that at least three enzymes were involved: an NADH-cytochrome-c reductase, an NADH-FeCN reductase, and an NAD(P)H-DCPIP and FeCN reductase. While the latter reductase had an acid pH optimum, indicating vacuolar origin, the NADH-cytochrome-c and FeCN reductases both had neutral pH optima, indicating cytoplasmic origin. Characterization of the NADH-specific reductases indicated that NADH-FeCN reductase may be a soluble form of the microsomal membrane NADH dehydrogenase but that NADH-cytochrome-c reductase may be normally soluble and possibly involved in cyanide-sensitive NADH oxidation.

The induced development of all three reductases was inhibited by 6-methylpurine, ethionine and cycloheximide, indicating dependence on both RNA and protein synthesis. The inhibition by cycloheximide could be reversed but this reversion required a 20-h washing-out period to be complete.

Keywords

Fractionation NADPH NADH Reductase Activity Cycloheximide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DCPIP

2,6-dichlorophenol indophenol

FeCN

ferricyanide

NO QNO

2-n-nonylhydroxyquinoline-N-oxide

pCMB

p-chloromercuribenzoate

SF

soluble fraction

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • J. M. Rungie
    • 1
  • J. T. Wiskich
    • 1
  1. 1.Department of BotanyUniversity of AdelaideAdelaideAustralia

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