Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Dispersed repeats in Drosophila virilis: elements mobilized by interspecific hybridization


A new class of moderately repetitive elements, designated the pDv sequences of Drosophila virilis, was cloned and molecularly analysed. The pDv elements occupy about 200 sites in the D. virilis genome. Their number decreases in related species with increasing phylogenetic distance. The pDv elements are characterized by a rather stable localization within the D. virilis genome, by intensive transcription and by partial polytenization in salivary gland chromosomes. Furthermore, they are capable of transposition in interspecific hybrids. DNA sequence analysis revealed that the pDv element contains tandemly arranged 36 bp repeat units which are flanked by imperfect direct terminal repeats of about 80 bp and inverted terminal repeats of 4 bp.

This is a preview of subscription content, log in to check access.


  1. Barnes WM, Bevan M (1983) Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition. Nucleic Acids Res 11:349–368

  2. Bonner JJ, Pardue ML (1976) Ecdysone stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Chromosoma 58:87–99

  3. Endow SA, Glover DM (1979) Differential replication of ribosomal gene repeats in polytene nuclei of Drosophila. Cell 17:597–605

  4. Evgen'ev MB, Kolchinsky A, Levin A, Preobrazhenskaya O, Sarkisova E (1978) Heat-shock homology in distantly related species of Drosophila. Chromosoma 68:357–365

  5. Evgen'ev MB, Polianskaya GG (1976) The pattern of polytene chromosome synapsis in Drosophila species and interspecific hybrids. Chromosoma 57:285–295

  6. Evgen'ev MB, Yenikolopov GN, Peunova NI, Ilyin YV (1982) Transposition of mobile genetic elements in interspecific hybrids of Drosophila. Chromosoma 85:375–386

  7. Evgen'ev MB, Zelentsova ES (1984) Evolution of dispersed genetic element and satellite DNA in Drosophila. Cytology and Genetics USSR 6:438–442

  8. Finnegan DJ, Rubin GM, Young HW, Hogness DS (1978) Repeated gene families in Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 42:1053–1064

  9. Flavell AJ, Ish-Horowicz D (1983) The origin of extrachromosomal circular copia element. Cell 34:415–419

  10. Gall JL, Atherton DD (1974) Satellite DNA sequences in D. virilis. J Mol Biol 85:633–664

  11. Gubenko IS, Baritcheva EM (1979) Drosophila virilis puffs induced by temperature and other environmental factors. Genetica USSR 15:1399–1414

  12. Gubenko IS, Evgen'ev MB (1984) Cytological and linkage maps of D. virilis chromosomes. Genetica 65:127–139

  13. Hennig W, Meer A (1971) Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature New Biol 233:70–72

  14. Ilyin YV, Tchurikov NA, Ananiev EV, Ryskov AP, Yenikolopov GN, Limborska SA, Maleeva NE, Gvozdev VA, Georgiev GP (1978) Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spring Harbor Symp Quant Biol 42:959–970

  15. Ilyin YV, Schuppe NG, Lyubomirskaya NV, Gorelova TV, Arkhipova IR (1984) Circular copies of mobile dispersed genetic elements in cultured Drosophila melanogaster cells. Nucleic Acids Res 12:7517–7531

  16. Kafatos FC, Jones CW, Efstratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization. Nucleic Acids Res 7:1541–1552

  17. Lifschytz E (1983) Sequence replication and banding organization in the polytene chromosomes of D. melanogaster. J Mol Biol 164:17–34

  18. Lummei J, Lankinen P (1981) Close to equal crossing-over frequencies in males and females in Drosophila littoralis. Hereditas 94:285–286

  19. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, NY

  20. Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564

  21. McGinnis W, Shermoen AW, Beckendorf SK (1983) A transposable element inserted just 5′ to a Drosophila glue protein genealters gene expression and chromatin structure. Cell 34:75–84

  22. Orgel LE, Crick FHC (1980) Selfish DNA: the ultimate parasite. Nature 284:604–607

  23. Patterson JT, Stone WS (1952) Evolution in the genus Drosophila. Macmillan Co., New York

  24. Potter SS (1982) DNA sequence of a foldback transposable element in Drosophila. Nature 297:201–204

  25. Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling DNA to high specific acivity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:239–251

  26. Rubin GM, Kidwell ML, Bingham PM (1982) The molecular basis of P — M hybrid dysgenesis: the nature of induced mutations. Cell 29:987–994

  27. Schmidt ER (1984) Clustered and interspersed repetitive DNA sequence family of Chironomus. The nucleotide sequence of the Cla-elements and of various flanking sequence. J Mol Biol 178:1–15

  28. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–518

  29. Staden R (1982) Automation of the computer handling of gel reading data produced by the shot-gun method of DNA sequencing. Nucleic Acids Res 10:4731–4751

  30. Throckmorton LH (1982) The virilis species group. In: Ashburner M et al. (eds) The genetics and biology of Drosophila, vol. 3b. Academic Press, London, pp 227–296

  31. Wensink PC, Tabata S, Pachl C (1979) The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA. Cell 18:1231–1246

  32. Yao MC, Choi J, Yokoyama Sh, Ching-Ho Yao (1984) DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA and definitive sites. Cell 36:433–440

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zelentsova, E.S., Vashakidze, R.P., Krayev, A.S. et al. Dispersed repeats in Drosophila virilis: elements mobilized by interspecific hybridization. Chromosoma 93, 469–476 (1986).

Download citation


  • Salivary Gland
  • Developmental Biology
  • Related Species
  • Repeat Unit
  • Interspecific Hybrid