Chromosoma

, Volume 93, Issue 6, pp 461–468 | Cite as

Electron microscopical analysis of Drosophila polytene chromosomes

IV. Mapping of morphological structures appearing as a result of transformation of DNA sequences into chromosomes
  • V. F. Semeshin
  • E. S. Belyaeva
  • I. F. Zhimulev
  • J. T. Lis
  • G. Richards
  • M. Bourouis
Article

Abstract

An electron microscopic (EM) analysis was performed on regions of Drosophila melanogaster polytene chromosomes that contain inserted DNA segments of 19 and 8 kb. These segments had been inserted by P-elementmediated transformation. The 19 kb segment includes both the Drosophila hsp70 gene fused to the Escherichia coli β-galactosidase gene and the rosy gene (Lis et al. 1983). This insert generates a new moderate-size band at the 9D4-9E1-2 region in polytene chromosomes. Upon heat shock, a puff originates from a portion of the new band. The 8 kb segment includes the Sgs7 and Sgs3 genes (Richards et al. 1983). This insert generates very diffuse thin bands that decondense at the stage of activation of the Sgs genes to produce wide interbands or small puffs. In all of the above cases, the insertion appears to occur at interband regions, and the genetically complex DNA segments that are inserted generate only a single detectable band.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • V. F. Semeshin
    • 1
  • E. S. Belyaeva
    • 1
  • I. F. Zhimulev
    • 1
  • J. T. Lis
    • 2
  • G. Richards
    • 3
  • M. Bourouis
    • 3
  1. 1.Siberian Division of the USSR Academy of SciencesInstitute of Cytology and GeneticsNovosibirskUSSR
  2. 2.Section of Biochemistry, Molecular and Cell BiologyCornell UniversityIthacaUSA
  3. 3.Laboratoire de Génétique Moléculaire des Eukaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERMInstitut de Chimie Biologique, Faculté de MédecineStrasbourg CédexFrance

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