Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Variation in the nitrate reductase of rice seedlings

  • 40 Accesses

  • 11 Citations


Nitrate reductase was induced in rice seedlings by nitrate and by chloramphenicol. During the induction period the different enzyme activities associated with nitrate reductase increased to different degrees. Nitrate induced high NADH-nitrate reductase activity and a great increase in the NADH-cytochrome c reductase activity which was associated with the nitrate reductase in a sucrose gradient. Chloramphenicol induced a nitrate reductase which had higher activity with NADPH than NADH. Chloramphenicol also induced a marked increase in NADPH-cytochrome c reductase activity as well as in NADH-cytochrome c reductase activity. Both activities were associated with the nitrate reductase in a sucrose gradient.

After partial purification by sucrose gradient sedimentation or by starch gel electrophoresis, the nitrate reductase of rice induced by nitrate and chloramphenicol showed the same preference in pyridine nucleotide cofactors as was shown by the crude enzyme extracts.

This is a preview of subscription content, log in to check access.


  1. Beevers, L., Flesher, D., Hageman, R. H.: Studies on the pyridine nucleotide specificity of nitrate reductase in higher plants and its relationship to sulfhydryl level. Biochim. biophys. Acta (Amst.) 89, 453–464 (1964).

  2. Garrett, R. H., Nason, A.: Further purification and properties of Neurospora nitrate reductase. J. biol. Chem. 244, 2870–2882 (1969).

  3. Martin, R. G., Ames, B. N.: A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J. biol. Chem. 236, 1372–1379 (1961).

  4. Notton, B. A., Hewitt, E. J.: The role of tungsten in the inhibition of nitrate reductase activity in spinach (Spinacea oleracea L.) leaves. Biochem. biophys. Res. Commun. 44, 702–710 (1971).

  5. Scandalios, J. G.: The genetic control of multiple molecular forms of enzymes in plants: a review. Biochem. Genet. 3, 37–79 (1969).

  6. Shen, T. C.: The induction of nitrate reductase and the preferential assimilation of ammonium in germinating rice seedlings. Plant Physiol. 44, 1650–1655 (1969).

  7. Shen, T. C.: Nitrate reductase of rice seedlings and its induction by organic nitrocompounds. Plant Physiol. 49, 546–549 (1972).

  8. Sims, A. P., Folkes, B. F., Bussey, A. H.: Mechanisms involved in the regulation of nitrogen assimilation in micro-organisms and plants. In: Recent aspect of nitrogen metabolism in plants, p. 91–114, Hewitt, E. J., Cutting, C. V., eds. New York: Acad. Press 1968.

  9. Sorger, G. J.: Nitrate reductase electron transport systems in mutant and in wildtype strains of Neurospora. Biochim. biophys. Acta (Amst.) 118, 484–494 (1966).

  10. van'T, Riet, J., Stouthamer, A. H., Planta, R. J.: Regulation of nitrate assimilation and nitrate respiration in Aerobacter aerogenes. J. Bact. 96, 1455–1464 (1968).

  11. Wray, J. L., Filner, P.: Structural and functional relationships of enzyme activities induced by nitrate in barley. Biochem. J. 119, 715–725 (1970).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shen, T.C. Variation in the nitrate reductase of rice seedlings. Planta 108, 21–28 (1972). https://doi.org/10.1007/BF00386503

Download citation


  • NADH
  • Chloramphenicol
  • Nitrate Reductase
  • Reductase Activity