Advertisement

Planta

, Volume 90, Issue 4, pp 349–359 | Cite as

Studies on the growth and flowering of a short-day plant, Wolffia microscopica

II. Role of metal ions and chelates
  • P. N. Seth
  • Rukmani Venkataraman
  • S. C. Maheshwari
Article

Summary

As found earlier, supply of EDTA was obligatory for both flowering and satisfactory vegetative growth in Wolffia microscopica. It is now shown that the metal affecting growth and flowering is most probably iron. Omission of Fe but not of Cu, Zn, Mn and B from the medium markedly affects vegetative growth. There exists also a strong interaction between EDTA and Fe, one being largely inactive in the absence of the other. When Fe-EDDHA is substituted for Fe-citrate and EDTA in the medium, no great effect is seen in vegetative growth, but flowering takes place even under continuous light. Studies with 59Fe show that, in the medium containing Fe-EDDHA, Fe uptake is stimulated several-fold; this is apparently associated with the flowering condition.

Keywords

Iron EDTA Flowering Condition Strong Interaction Vegetative Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

EDTA

ethylenediaminetetraacetic acid

EDDHA

ethylenediamine-di-o-hydroxyphenylacetic acid

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Frost, A. E., Freedman, H. H., Westerback, S. J., Martell, A. E.: Chelating tendencies of N, N-ethylenebis-[2-(o-hydroxyphenyl)]-glycine. J. Amer. chem. Soc. 80, 530–536 (1958).Google Scholar
  2. Hillman, W. S.: Photoperiodism, chelating agents, and flowering of Lemna perpusilla and L. gibba in aseptic culture. In: Light and life (W. D. McElroy and B. Glass, eds.), p. 673–686. Baltimore: Johns Hopkins Press 1961 (a).Google Scholar
  3. —: Experimental control of flowering in Lemna. III. A relationship between medium composition and the opposite photoperiodic responses of L. perpusilla 6746 and L. gibba G3. Amer. J. Bot. 48, 413–419 (1961b).Google Scholar
  4. —: The Lemnaceae, or duckweeds. Bot. Rev. 27, 221–287 (1961c).Google Scholar
  5. —: Experimental control of flowering in Lemna. IV. Inhibition of photoperiodic sensitivity by copper. Amer. J. Bot. 49, 892–897 (1962).Google Scholar
  6. Holmes, R. S.: Determination of total copper, zinc, cobalt, and lead in soils and soil solutions. Soil Sci. 59, 77–84 (1945).Google Scholar
  7. Kroll, H., Knell, M., Powers, J., Simonian, J.: A phenolic analogue of ethylenediamine tetraacetic acid. J. Amer. chem. Soc. 79, 2024–2025 (1957).Google Scholar
  8. Maheshwari, S. C., Chauhan, O. S.: In vitro control of flowering in Wolffia microscopia. Nature (Lond.) 198, 99–100 (1963).Google Scholar
  9. —, Seth, P. N.: Induction of flowering in Wolffia microscopica by the iron salt of ethylenediamine-di-o-hydroxyphenylacetic acid (Fe-EDDHA). Z. Pflanzenphysiol. 55, 89–91 (1966).Google Scholar
  10. —, Venkataraman, R.: Induction of flowering in a duckweed, Wolffia microscopica, by a new kinin, zeatin. Planta (Berl.) 70, 304–306 (1966).Google Scholar
  11. Munns, D. N., Johnson, C. M.: Removal of heavy metal and halide contamination from macronutrient salts. Plant Physiol. 65, 978–981 (1960).Google Scholar
  12. Smith, G. F., McCurdy, W. H., Jr., Diehl, H.: The colorimetric determination of iron in raw and treated municipal water supplies by use of 4:7-diphenyl-1:10-phananthroline. Analyst 77, 418–422 (1952).Google Scholar
  13. Smith, H. J., McIlrath, W. J., Bogorad, L.: Some effects of iron deficiency on flowering of Xanthium. Bot. Gaz. 118, 174–179 (1957).Google Scholar
  14. Venkataraman, R., Seth, P. N., Maheshwari, S. C.: Studies on the growth and flowering of a short-day plant, Wolffia microscopica. I. General aspects and induction of flowering by cytokinins. Z. Pflanzenphysiol. (in press).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • P. N. Seth
    • 1
  • Rukmani Venkataraman
    • 1
  • S. C. Maheshwari
    • 1
  1. 1.Department of BotanyUniversity of DelhiIndia

Personalised recommendations