Planta

, Volume 104, Issue 4, pp 357–361 | Cite as

The use of nurse culture in the development of haploid clones in tomato

  • W. R. Sharp
  • R. S. Raskin
  • H. E. Sommer
Short Communication

Summary

A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussed

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • W. R. Sharp
    • 1
  • R. S. Raskin
    • 1
  • H. E. Sommer
    • 2
  1. 1.Department of MicrobiologyOhio State UniversityColumbusUSA
  2. 2.Department of BiochemistryOhio State UniversityColumbusUSA

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