, Volume 104, Issue 4, pp 357–361 | Cite as

The use of nurse culture in the development of haploid clones in tomato

  • W. R. Sharp
  • R. S. Raskin
  • H. E. Sommer
Short Communication


A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussed


Pollen Cell Anther Culture Skoog Medium Culture Procedure Nurse Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abo El-Nil, M. M., Hildebrandt, A. C.: Induction of geranium plants from anther callus. In Vitro 7, 399 (1971).Google Scholar
  2. Clapham, D.: In vitro development of callus from the pollen of Lolium and Hordeum. Z. Pflanzenzüchtg. 65, 285–292 (1971).Google Scholar
  3. Devreux, M., Saccardo, F., Brunori, A.: Plantes haploides et lignes isogeniques de Nicotiana tabacum obtenues par cultures d'anthères et tiges in vitro. Caryologia 24, 141–148 (1971).Google Scholar
  4. Guha, S., Maheshwari, S. C.: Development of embryoids from pollen grains of Datura in vitro. Phytomorphology 17, 454–461 (1967).Google Scholar
  5. Kameya, T., Hinata, K.: Induction of haploid plants from pollen grains of Brassica. Jap. J. Breeding 20, 82–87 (1970).Google Scholar
  6. Melchers, G., Bergmann, L.: Untersuchungen an Kulturen von haploiden Geweben von Antirrhinum majus. Ber. dtsch. bot. Ges. 71, 459–473 (1959).Google Scholar
  7. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum (Oph.) 15, 473–497 (1962).Google Scholar
  8. Nickell, L. G., Torrey, J. G.: Crop improvement through plant cell and tissue culture. Science 166, 1068–1069 (1969).Google Scholar
  9. Niizeki, H., Oono, K.: Induction of haploid rice plants from anther culture. Proc. Japan Acad. 44, 554–557 (1968).Google Scholar
  10. Nishi, T., Mitsuoka, S.: Plants from anther and ovary culture of rice plant. Jap. J. Genet. 44, 341–346 (1969).Google Scholar
  11. Nitsch, J. P., Nitsch, C.: Haploid plants from pollen grains. Science 163, 85–87 (1969).Google Scholar
  12. Sharp, W. R., Dougall, D. K., Paddock, E. F.: Haploid plantlets and callus from immature pollen grains of Nicotiana and Lycopersicon. Bull. Torrey bot. Club 98, 219–222 (1971).Google Scholar
  13. Sharp, W. R., Raskin, R. S., Sommer, H. E.: Haploidy in Lilium. Phytomorphology (in press) (1972).Google Scholar
  14. Van't Hof, J.: Experimental procedures for measuring cell population kinetic parameters in plant root meristems. In: Methods in cell physiology, D. Prescott, ed., vol. III, p. 95–117. New York: Acad. Press 1968.Google Scholar
  15. White, P. R.: The cultivation of animal and plant cells. New York: Ronald Press 1963.Google Scholar
  16. Zenkteler, M.: In vitro production of haploid plants from pollen grains of Atropa belladonna L. Experientia (Basel) 27, 1087 (1971).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • W. R. Sharp
    • 1
  • R. S. Raskin
    • 1
  • H. E. Sommer
    • 2
  1. 1.Department of MicrobiologyOhio State UniversityColumbusUSA
  2. 2.Department of BiochemistryOhio State UniversityColumbusUSA

Personalised recommendations