Pflügers Archiv

, Volume 430, Issue 5, pp 802–807

Polarized ion transport during migration of transformed Madin-Darby canine kidney cells

  • Albrecht Schwab
  • Kerstin Gabriel
  • Franz Finsterwalder
  • Gunnar Folprecht
  • Rainer Greger
  • Albrecht Kramer
  • Hans Oberleithner
Original Article Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

DOI: 10.1007/BF00386179

Cite this article as:
Schwab, A., Gabriel, K., Finsterwalder, F. et al. Pflugers Arch. (1995) 430: 802. doi:10.1007/BF00386179

Abstract

Epithelial cells lose their usual polarization during carcinogenesis. Although most malignant tumours are of epithelial origin little is known about ion channels in carcinoma cells. Previously, we observed that migration of transformed Madin-Darby canine kidney (MDCK-F) cells depended on oscillating K+ channel activity. In the present study we examined whether periodic K+ channel activity may cause changes of cell volume, and whether K+ channel activity is distributed in a uniform way in MDCK-F cells. After determining the average volume of MDCK-F cells (2013±270 μm3; n=8) by means of atomic force microscopy we deduced volume changes by calculating the K+ efflux during bursts of K+ channel activity. Therefore, we measured the membrane conductance of MDCK-F cells which periodically rose by 22.3±2.5 nS from a resting level of 6.5±1.4 nS (n=12), and we measured the membrane potential which hyperpolarized in parallel from −35.4±1.2 mV to −71.6±1.8 mV (n=11). The distribution of K+ channel activity was assessed by locally superfusing the front or rear end of migrating MDCK-F cells with the K+ channel blocker charybdotoxin (CTX). Only exposure of the rear end to CTX inhibited migration providing evidence for “horizontal” polarization of K+ channel activity in transformed MDCK-F cells. This is in contrast to the “vertical” polarization in parent MDCK cells. We propose that the asymmetrical distribution of K+ channel activity is a prerequisite for migration of MDCK-F cells.

Key words

MDCK-F cell Polarization K+ channel Cell volume Atomic force microscopy 

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Albrecht Schwab
    • 1
  • Kerstin Gabriel
    • 1
  • Franz Finsterwalder
    • 1
  • Gunnar Folprecht
    • 1
  • Rainer Greger
    • 2
  • Albrecht Kramer
    • 2
  • Hans Oberleithner
    • 1
  1. 1.Physiologisches InstitutWürzburgGermany
  2. 2.Physiologisches InstitutFreiburgGermany

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