, Volume 2, Issue 2, pp 85–94 | Cite as

Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study

  • Sankar Sen
  • Friedrich Srienc
  • Wei-Shou Hu


Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.

Key words

hybridoma cell volume cell culture flow cytometry 


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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Sankar Sen
    • 1
  • Friedrich Srienc
    • 1
    • 2
  • Wei-Shou Hu
    • 1
  1. 1.Department of Chemical Engineering and Materials ScienceUniversity of MinnesotaMinneapolisUSA
  2. 2.Institute for Advanced Studies in Biological Process TechnologyUniversity of MinnesotaSt. PaulUSA

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