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Planta

, Volume 108, Issue 2, pp 93–102 | Cite as

Concerning the presence of the cytokinin, N6-(Δ2-isopentnyl) adenine, in cultures of Corynebacterium fascians

  • Michel P. Rathbone
  • Ross H. Hall
Article

Summary

Corynebacterium fascians causes a fasciation disease in a number of dicotyledons and this disease appears to be caused by compounds with cytokinin activity elaborated by the infecting bacteria. Extractions of C. fascians in late logarithmic phase under conditions where the pH never falls below 7.0 yield about 2 μg/l of N6-(Δ2, a potent cytokinin. If a mild acidification step is included in the extraction procedure the yield increases to about 12 μg/l. This is due to release of N6-(Δ2-isopentenyl)adenine from C. fascians tRNA during the extraction procedure. In terms of total cytokinin activity present in C. fascians cultures, N6-(Δ2-isopentenyl)adenine appears to be a minor component.

Keywords

Adenine Extraction Procedure Minor Component Corynebacterium Logarithmic Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alam, S. N., Hall, R. H.: Separation of the 101-1 and 101-2 isomers of 101-3 by gas chromatography and characterization by mass spectroscopy. Analyt. Biochem. 40, 424–428 (1971).Google Scholar
  2. Armstrong, D. J., Burrows, W. J., Evans, P. K., Skoog, F.: Isolation of cytokinins from tRNA. Biochem. biophys. Res. Commun. 37, 451–456 (1969).Google Scholar
  3. Dyson, W. H., Chen, C. M., Alam, S. N., Hall, R. H., Hong, C. I., Chheda, G. B.: Cytokinin activity of ureidopurine derivatives related to a modified nucleoside found in transfer RNA. Science 170, 328–330 (1970).Google Scholar
  4. Girard, M.: Isolation of ribonucleic acids from mammalian cells and animal viruses. Meth. in Enzymology 12 (Part A), 581–588 (1967).Google Scholar
  5. Hall, R. H.: The modified nucleosides in nucleic acids. New York: Columbia University Press 1971.Google Scholar
  6. Hall, R. H., Srivastava, B. I. S.: Cytokinin activity of compounds obtained from soluble RNA. Life Sci. 7 (Part 2), 7–13 (1968).Google Scholar
  7. Klämbt, D., Thies, G., Skoog, F.: Isolation of cytokinins from Corynebacterium fascians. Proc. nat. Acad. Sci. (Wash.) 56, 52–59 (1966).Google Scholar
  8. Martin, D. M. G., Reese, C. B.: Some aspects of the chemistry of N(1)- and N(6)-dimethylallyl derivatives of adenosine and adenine. J. chem. Soc. (C), 1731–1738 (1968).Google Scholar
  9. Matsubara, S., Armstrong, D. J., Skoog, F.: Cytokinins in tRNA of Corynebacterium fascians. Plant Physiol. 43, 451–453 (1968).Google Scholar
  10. Robins, M. J., Hall, R. H., Thedford, R.: 102-1. A component of the tRNA of yeast and of mammalian tissue, methods of isolation, and characterization. Biochemistry 6, 1837–1848 (1967).Google Scholar
  11. Roussaux, J.: Etude préliminaire des modifications induites chez le pois Express Alaska par le Corynebacterium fascians (Tilford) Dowson. Rev. gén. Bot. 72, 21–53 (1965)Google Scholar
  12. Thimann, K. V., Sachs, T.: The role of cytokinins in the “fasciation” disease caused by Corynebacterium fascians. Amer. J. Bot. 53, 731–739 (1966).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Michel P. Rathbone
    • 1
  • Ross H. Hall
    • 1
  1. 1.Department of BiochemistryMcMaster UniversityHamiltonCanada

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