Molecular and General Genetics MGG

, Volume 102, Issue 3, pp 229–231 | Cite as

Ethyl methanesulfonate: an effective mutagen inChlamydomonas reinhardi

  • R. Loppes


The effectiveness of ethyl methanesulfonate (EMS) as a mutagen inChlamydomonas reinhardi has been demonstrated in two different systems: reversion to prototrophy of an argine-requiring strain and forward mutation to auxotrophy of the wild-type strain. In these experiments, the toxic effect of EMS was very low. With certain batches of EMS, an unusually high toxicity was observed. In this case, the lethal effects seem to be attributable to the presence of minor impurities in EMS.


Toxicity Ethyl Toxic Effect High Toxicity Lethal Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Froese-Gertzen, E. E., C. F.Konzak, R. J.Foster, and R. A.Nilan: Correlation between some chemical and biological reactions of ethyl methanesulfonate. Nature (Lond.)198, 447 (1963).Google Scholar
  2. Lindegren, G., H. L.Hwang, Y.Oshima, and C. C.Lindegren: Genetical mutants induced by ethyl methanesulfonate inSaccharomyces. Canad. J. Genet. Cytol.7, 491–499 (1965).Google Scholar
  3. Loppes, R.: Nature du milieu sélectif et expression phénotypique des mutations induites par le méthane sulfonate d'éthyl (EMS) chezChlamydomonas reinhardi. Ann. Inst. Pasteur110, 645–654 (1966a).Google Scholar
  4. —: Damage induced by methyl methanesulfonate (MMS) inChlamydomonas reinhardi. Z. Vererbungsl.98, 193–202 (1966b).Google Scholar
  5. Loveless, A., and S.Howarth: Mutation of bacteria at high levels of survival by ethyl methanesulphonate. Nature (Lond.)184, 1780–1782 (1959).Google Scholar
  6. Rannenberg, H., u. C. G.Arnold: Die Wirkung von Äthylmethansulfonat aufChlamydomonas reinhardi. Molec. Gen. Genetics101, 212–216 (1968).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • R. Loppes
    • 1
  1. 1.Laboratory of GeneticsUniversity of LiègeBelgium

Personalised recommendations