Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Die Phenylalaninammoniumlyase (PAL, EC 4.3.1.5) des Senfkeimlings (Sinapis alba L.), ein elektrophoretisch einheitliches Enzym

Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) of the mustard seedling (Sinapis alba L.), an electrophoretically homogeneous enzyme

  • 37 Accesses

  • 15 Citations

Summary

Improved techniques in localization of phenylalanine ammonia-lyase (PAL) on polyacrylamide disk electrophoresis columns indicate that the enzyme synthesized under the control of phytochrome is electrophoretically indistinguishable from the enzyme present in dark grown mustard seedlings. Furthermore, no heterogeneity of PAL with respect to molecular size has been detected. However, the formation of high molecular weight aggregates with PAL activity in tris buffer of low concentration has been demonstrated. The data lead to the conclusion that phytochrome does not induce the synthesis of a novel PAL enzyme differing in its structural properties from the PAL in dark grown seedlings. The observations of other investigators on separable forms of PAL are critically discussed.

This is a preview of subscription content, log in to check access.

Literatur

  1. Ahmed, S. I., Swain, T.: The effect of light on the activity of enzymes of the aromatic pathway in peas and mung beans. Phytochemistry9, 2287–2290 (1970).

  2. Amrhein, N., Zenk, M. H.: Untersuchungen zur Rolle der Phenylalanin-Ammonium-Lyase (PAL) bei der Regulation der Flavonoidsynthese im Buchweizen (Fagopyrum esculentum Moench). Z. Pflanzenphysiol.64, 145–168 (1971).

  3. Davis, B. J.: Disc electrophoresis — II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci.121, Art. 2, 404–427 (1964).

  4. Emes, A. V., Vining, L. C.: Partial purification and properties of L-phenylalanine ammonia-lyase fromStreptomyces verticillatus. Canad. J. Biochem.48, 613–622 (1970).

  5. Havir, E. A., Hanson, K. R.: L-phenylalanine ammonia-lyase. I. Purification and molecular size of the enzyme from potato tubers. Biochemistry7, 1896–1903 (1968).

  6. Marsh, H. V., Jr., Havir, E. A., Hanson, K. R.: L-phenylalanine ammonia-lyase. III. Properties of the enzyme from maize seedlings. Biochemistry7, 1915–1918 (1968).

  7. Minamikawa, T., Uritani, I.: Phenylalanine ammonia-lyase in sliced sweet potato roots. J. Biochem. (Tokyo)57, 678–688 (1965).

  8. Mohr, H.: Untersuchungen zur phytochrominduzierten Photomorphogenese des Senfkeimlings (Sinapis alba L.). Z. Pflanzenphysiol.54, 63–83 (1966).

  9. — Huault, C., Lange, H., Lohmann, L., Rissland, I., Weidner, M.: Lag-phases in phytochrome-mediated enzyme synthesis (PAL). Planta (Berl.)83, 267–275 (1968).

  10. Rissland, I., Mohr, H.: Phytochrom-induzierte Enzymbildung (Phenylalanindesaminase), ein schnell ablaufender Prozeß. Planta (Berl.)77, 239–249 (1967).

  11. Schopfer, P., Hock, B.: Nachweis der Phytochrom-induziertende novo-Synthese von Phenylalaninammoniumlyase (PAL, E.C. 4.3.1.5) in Keimlingen vonSinapis alba L. durch Dichtemarkierung mit Deuterium. Planta (Berl.)96, 248–253 (1971).

  12. Stafford, H. A.: Changes in phenolic compounds and related enzymes in young plants ofSorghum. Phytochemistry8, 743–752 (1969).

  13. Weidner, M., Jakobs, M., Mohr, H.: Über den Einfluß des Phytochroms auf den Gehalt an Ribonucleinsäure und Protein in Senfkeimlingen (Sinapis alba L.). Z. Naturforsch.20 b, 689–693 (1965).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Schopfer, P. Die Phenylalaninammoniumlyase (PAL, EC 4.3.1.5) des Senfkeimlings (Sinapis alba L.), ein elektrophoretisch einheitliches Enzym. Planta 99, 339–346 (1971). https://doi.org/10.1007/BF00385825

Download citation