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Humangenetik

, Volume 20, Issue 3, pp 195–202 | Cite as

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

Assignment of PGM3 to chromosome C6 and regional mapping of the PGD, PGM1 and Pep-C genes on chromosome A1
  • Ans Jongsma
  • Harry van Someren
  • Andries Westerveld
  • Ann Hagemeijer
  • Peter Pearson
Original Investigations

Summary

About 75 man-Chinese hamster hybrid clones were analysed for their human chromosome complement and simultaneously tested for human enzyme markers. Correlation of the presence of chromosomes and enzyme activity revealed assignments of the PGD linkage group to chromosome 1, ME1, PGM3 and IPO-B to 6, LDH-A to 11, LDH-B to 12 and IPO-A to 21.

The assignment of PGM3 puts the HL-A loci on chromosome 6. Segregation of the enzymes of the PGD linkage group was demonstrated in a clone which had retained a deleted chromosome 1. Subclones of this line indicate that the loci for PGD and PGM1 are situated on the short arm or proximal part of the long arm of 1 and the locus for Pep-C on the long arm.

Keywords

Metabolic Disease Somatic Cell Human Chromosome Proximal Part Cell Hybrid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Etwa 75 Hybrid-Zellklone Mensch/Chinesischer Hamster wurden in bezug auf den menschlichen Anteil ihres Chromosomensatzes analysiert und gleichzeitig auf menschliche Enzym-Marker untersucht. Die Korrelation zwischen Anwesenheit von Chromosomen und Enzym-Markern ließ die Folgerung zu, daß die PGD-Koppelungsgruppe auf Chromosom 1, ME1, PGM3 und IPO-B auf Nr. 6, LDH-A auf 11, LDH-B auf 12 und IPO-A auf Chromosom 21 gelegen ist.

Die Lokalisation von PGM3 läßt die Folgerung zu, daß auch die HL-A-loci auf Chromosom 6 lokalisiert sind. Aufspaltung der Enzyme der PGD-Koppelungsgruppe konnte an einem Klon dargestellt werden, der ein deletiertes Chromosom 1 enthielt. Die Subklone dieser Linie zeigen, daß die loci für PGD und PGM1 auf dem kurzen Arm oder dem proximalen Teil des langen Arms von Chromosom Nr. 1 liegen, während der locus für Pep-C auf dem langen Arm gelegen ist.

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References

  1. Billardon, C., van Cong, N., Picard, J. Y., Le Borgne de Kaouël, Ch., Rebourcet, R., Weil, D., Feingold, J., Frézal, J.: Linkage studies of enzyme markers in man-mouse somatic cell hybrids. Ann. hum. Genet.36, 273–284 (1973)Google Scholar
  2. Boone, Ch., Chen, T. R., Ruddle, F. H.: Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17 and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids. Proc. nat. Acad. Sci. (Wash.)69, 510–514 (1972)Google Scholar
  3. Bootsma, D., Keijzer, W., Kleijer, W. J., de Weerd-Kastelein, E. A., de Wit, J., Meera Khan, P., van Someren, H., Westerveld, A.: Mammalian cell culture studies. Genetics73, Suppl., 167–179 (1973)Google Scholar
  4. Burgerhout. W., van Someren, H., Bootsma, D.: Cytological mapping of the genes assigned to the human Al chromosome by use of radiation-induced chromosome breakage in a human-Chinese hamster hybrid cell line. Humangenetik20, 159–162 (1973)Google Scholar
  5. Caspersson, T., Zech, L., Johansson, C.: Analysis of human metaphase chromosome set by aid of DNA binding fluorescent agents. Exp. Cell Res.62, 490–492 (1970)Google Scholar
  6. Chen, T. R., Mc Morris, F. A., Creagan, R., Ricciuti, F., Tischfield, J., Ruddle, F. H.: Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man. Amer. J. hum. Genet.25, 200–207 (1973)Google Scholar
  7. Cong, N. van, Billardon, C., Picard, J. Y., Feingold, J., Frézal, J.: Liaison probable (linkage) entre les locus PGM1 and peptidase C chez l'homme. C. R. Acad. Sci. (Paris)272, 485–487 (1971)Google Scholar
  8. Douglas, G. R., McAlpine, Ph. J., Hamerton, J. L.: Regional localization of the loci for human PGM1 and 6PGD on human chromosome one using Chinese hamster-human somatic cell hybrids. Proc. nat. Acad. Sci. (Wash.)70, 2737–2740 (1973)Google Scholar
  9. Edwards, J. E., Allen, F. H., Glenn, K. P., Lamm, L. U., Robson, E. B.: The linkage relationships of HL-A. In: Histocompatibility testing, 1972, Dausset, J., Colombani, J., Eds., pp. 745–751. Copenhagen: Munksgaard 1973Google Scholar
  10. Hamerton, J. L., Douglas, G. R., Gee, Ph. A., Richardson, B. J.: The association of glucose phosphate isomerase expression with human chromosome 19 using somatic cell hybrids. Cytogenet. Cell Genet.12, 128–135 (1973)Google Scholar
  11. Hors-Cayla, M. C., Fellous, M., Piccard, J. Y., Trébuchet, C., Dauset, J., Frézal, J.: Liaison entre le système d'histocompatibilité HL-A et l'activité thymidine-kinase dans un hybride homme-souris. C. R. Acad. Sci. (Paris)275, 2965–2968 (1972)Google Scholar
  12. Lamm, L. U., Svejgaard, A., Kissmeyer-Nielsen, F.: PGM3: HL-A is another linkage in man. Nature (Lond.) New Biol.231, 109–110 (1971)Google Scholar
  13. Littlefield, J. W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science145, 709–710 (1964)Google Scholar
  14. Meera Khan, P.: Enzyme electrophoresis on cellulose acetate gel zymogram patterns in manmouse and man-Chinese hamster somatic cell hybrids. Arch. Biochem. Biophys.145, 470–483 (1971)Google Scholar
  15. Pearson, P. L.: The use of new staining techniques for human chromosome identification. J. med. Genet.9, 264–275 (1972)Google Scholar
  16. Renwick, J. H.: The mapping of human chromosomes. Ann. Rev. Genet.5, 81–119 (1971)Google Scholar
  17. Ricciuti, F., Ruddle, F. H.: Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci in man by somatic cell genetics. Nature (Lond.) New Biol.241, 180–182 (1973)Google Scholar
  18. Robson, E. B., Cook, P. J. L., Corney, G., Hopkinson, D. A., Noades, J., Cleghorn, T. E.: Linkage data on Rh, PGM1, PGD, peptidase C and Fy from family studies. Ann. hum. Genet.36, 393–399 (1973)Google Scholar
  19. Ruddle, F. H.: Linkage analysis in man by somatic cell genetics. Nature (Lond.)242, 165–169 (1973)Google Scholar
  20. Ruddle, F., Ricciuti, F., Mc Morris, F. A., Tischfield, J., Creagan, R., Darlington, G., Chen, T.: Somatic cell genetic assignment of peptidase C and the Rh linkage group to chromosome A-1 in man. Science176, 1429–1431 (1972)Google Scholar
  21. Sumner, A. T., Evans, H. J., Buckland, R. A.: A new technique for distinguishing between human chromosomes. Nature (Lond.) New Biol.232, 31–32 (1971)Google Scholar
  22. Someren, H. van, Westerveld, A., Hagemeijer, A., Mees, J. R., Meera Khan, P., Zaalberg, O. B.: Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids. Evidence for synteny between the HL-A, PGM3, ME1 and IPO-B loci. Proc. nat. Acad. Sci. (Wash.) (submitted, 1973)Google Scholar
  23. Tan, Y. H., Tischfield, J., Ruddle, F. H.: The linkage of genes for the human interferon induced antiviral protein and indophenol oxydase-B traits to chromosome G21. J. exp. Med.137, 317–330 (1973)Google Scholar
  24. Tischfield, J. A., Creagan, R. P., Ruddle, F. H.: Unpublished resultsGoogle Scholar
  25. Westerveld, A., Meera Khan, P.: Evidence for linkage between human loci for 6-phosphogluconate dehydrogenase and phosphoglucomutase 1 in man-Chinese hamster somatic cell hybrids. Nature (Lond.)236, 30–32 (1972)Google Scholar
  26. Westerveld, A., Meera Khan, P., Visser, R. P. L. S., Bootsma, D.: Loss of human genetic markers in man-Chinese hamster somatic cell hybrids. Nature (Lond.) New Biol234, 20–24 (1971)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Ans Jongsma
    • 1
  • Harry van Someren
    • 2
  • Andries Westerveld
    • 3
  • Ann Hagemeijer
    • 1
  • Peter Pearson
    • 4
  1. 1.Department of Cell Biology and GeneticsErasmus UniversityRotterdam
  2. 2.Medical Biological Laboratory TNO and Laboratory of Molecular GeneticsState University LeidenBijswijk
  3. 3.Medical Biological LaboratoryTNORijswijk
  4. 4.Department of Human GeneticsState University LeidenLeidenThe Netherlands

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