, Volume 20, Issue 3, pp 195–202 | Cite as

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

Assignment of PGM3 to chromosome C6 and regional mapping of the PGD, PGM1 and Pep-C genes on chromosome A1
  • Ans Jongsma
  • Harry van Someren
  • Andries Westerveld
  • Ann Hagemeijer
  • Peter Pearson
Original Investigations


About 75 man-Chinese hamster hybrid clones were analysed for their human chromosome complement and simultaneously tested for human enzyme markers. Correlation of the presence of chromosomes and enzyme activity revealed assignments of the PGD linkage group to chromosome 1, ME1, PGM3 and IPO-B to 6, LDH-A to 11, LDH-B to 12 and IPO-A to 21.

The assignment of PGM3 puts the HL-A loci on chromosome 6. Segregation of the enzymes of the PGD linkage group was demonstrated in a clone which had retained a deleted chromosome 1. Subclones of this line indicate that the loci for PGD and PGM1 are situated on the short arm or proximal part of the long arm of 1 and the locus for Pep-C on the long arm.


Metabolic Disease Somatic Cell Human Chromosome Proximal Part Cell Hybrid 
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Etwa 75 Hybrid-Zellklone Mensch/Chinesischer Hamster wurden in bezug auf den menschlichen Anteil ihres Chromosomensatzes analysiert und gleichzeitig auf menschliche Enzym-Marker untersucht. Die Korrelation zwischen Anwesenheit von Chromosomen und Enzym-Markern ließ die Folgerung zu, daß die PGD-Koppelungsgruppe auf Chromosom 1, ME1, PGM3 und IPO-B auf Nr. 6, LDH-A auf 11, LDH-B auf 12 und IPO-A auf Chromosom 21 gelegen ist.

Die Lokalisation von PGM3 läßt die Folgerung zu, daß auch die HL-A-loci auf Chromosom 6 lokalisiert sind. Aufspaltung der Enzyme der PGD-Koppelungsgruppe konnte an einem Klon dargestellt werden, der ein deletiertes Chromosom 1 enthielt. Die Subklone dieser Linie zeigen, daß die loci für PGD und PGM1 auf dem kurzen Arm oder dem proximalen Teil des langen Arms von Chromosom Nr. 1 liegen, während der locus für Pep-C auf dem langen Arm gelegen ist.


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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Ans Jongsma
    • 1
  • Harry van Someren
    • 2
  • Andries Westerveld
    • 3
  • Ann Hagemeijer
    • 1
  • Peter Pearson
    • 4
  1. 1.Department of Cell Biology and GeneticsErasmus UniversityRotterdam
  2. 2.Medical Biological Laboratory TNO and Laboratory of Molecular GeneticsState University LeidenBijswijk
  3. 3.Medical Biological LaboratoryTNORijswijk
  4. 4.Department of Human GeneticsState University LeidenLeidenThe Netherlands

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