Planta

, Volume 131, Issue 3, pp 235–243

The effect of ethylene on root meristems of Pisum sativum and Zea mays

  • P. W. Barlow
Article

Summary

Ethylene at a concentration of 100 μl l−1 causes a slight increase in the duration of the mitotic cycle in the primary root meristems of both Pisum sativum L. and Zea mays L. This is due to a lengthening of the G1 phase; other phases of the cycle are unaffected. Autoradiography and microdensitometry show that the rate of 3H-thymidine incorporation into nuclei of Pisum is maximal when about half the DNA has been replicated, and that ethylene has no effect upon this rate. Ethylene causes a reduction of the number of dividing cells in the root meristem, particularly in Pisum.

Abbreviations

\(T_S ,T_{G_1 } ,T_{G_2 }\)

Duration of the S phase, the G1 phase, the G2 phase of the mitotic cell cycle, respectively

TC

Duration of the complete mitotic cell cycle

QC

Quiescent centre

LI, MI

Labelling index, Mitotic index (i.e. fraction of the population labelled or in mitosis, respectively)

PF

Proliferative fraction (i.e. fraction of the population making progress towards mitosis)

[3H]dT

tritiated thymidine

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • P. W. Barlow
    • 1
  1. 1.Agricultural Research Council Unit of Developmental BotanyUniversity of CambridgeCambridgeUK

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