Regulation of RNA synthesis in higher plant cells by the action of a nucleoside triphosphatase
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Abstract
The influence of nucleoside triphosphates in relation to divalent cations on RNA synthesis of cells from a suspension culture from parsley was investigated. The data obtained from experiments with isolated nuclei and with an in vitro system with highly purified RNA polymerase I were compared with a chromatin-bound nucleoside triphosphatase activity within the nucleus. The results might suggest a regulatory role of the nucleoside triphosphatase activity in RNA synthesis.
Key words
Cell suspension culture Nuclei (isolated) Nucleoside triphosphatase RNA polymerase I RNA synthesis (regulation)Abbreviations
- NTP
nucleoside triphosphates
- NTPase
nucleoside triphosphatase
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References
- Grossmann, K., Friedrich, H., Seitz, H.U. (1980) Purification and characterization of chromatin-bound DNA-dependent RNA polymerase I from parsley (Petroselinum crispum). Influence of nucleoside triphosphates. Biochem. J. 191, 165–171Google Scholar
- Grossmann, K., Haschke, H.-P., Seitz, U., Seitz, U. (1979b) Synthesis and release of RNA by isolated nuclei of plant cells in relation to nucleoside triphosphates and divalent cations. Plant Syst. Evol. Suppl. 2, 163–177Google Scholar
- Grossmann, K., Seitz, U. (1979) RNA polymerase I from higher plants. Evidence for allosteric regulation and interaction with a nuclear phosphatase activity controlled NTP pool. Nucl. Acids Res. 7, 2015–2029Google Scholar
- Grossmann, K., Seitz, U., Seitz, H.U. (1979a) Transcription and release of RNA in isolated nuclei from parsley cells. Z. Naturforsch. 34c, 431–435Google Scholar
- Grummt, I., Grummt, F. (1976) Control of nucleolar RNA synthesis by the intracellular pool sizes of ATP and GTP. Cell 7, 447–453Google Scholar
- Guilfoyle, T.J., Hanson, J.B. (1973) Increased activity of chromatin-bound ribonucleic acid polymerase from soybean hypocotyl with spermidine and high ionic strength. Plant Physiol. 51, 1022–1025Google Scholar
- Libanti, C.M., Tandler, C.J. (1969) The distribution of the water-soluble inorganic orthophosphate within the cell. Accumulation in the nucleus. J. Cell Biol. 42, 754–765Google Scholar
- Lin, T., Morales, M.F. (1977) Application of a one-step procedure for measuring inorganic phosphate in the presence of proteins. The actomyosin ATPase system. Anal. Biochem. 77, 10–17PubMedGoogle Scholar
- Seitz, U., Richter, G. (1970) Isolierung und Charakterisierung schnell markierter, hochmolekularer RNA aus frei suspendierten Calluszellen der Petersilie (Petroselinum sativum). Planta 92, 309–326Google Scholar
- Siebert, G. (1968) Nucleus. In: Comprehensive biochemistry vol. 23, pp. 1–37. Florkin, M., Stotz, E.H. eds. Elsevier Publishing Company, Amsterdam New York LondonGoogle Scholar
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