A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
D'Alessio, G., Trim, A. R.: A method for the isolation of nucleic from leaves. J. exp. Bot. 19, 831–839 (1968)
Edmonds, M.: A cytidine triphosphate polymerase from thymus nuclei. J. biol. Chem. 240, 4621–4628 (1965)
Giles, K. W., Myers, A.: An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature (Lond.) 206, 93 (1965)
Hamilton, R. H., Künsch, U., Temperli, A.: Simple rapid procedures for isolation of tobacco leaf nuclei. Analyt. Biochem. 49, 48–57 (1972)
Hartmann, J. X., Kao, K. N., Gamborg, O. L., Miller, R. A.: Immunological methods for the agglutination of protoplasts from cell suspension cultures of different genera. Planta (Berl.) 112, 45–56 (1973)
Hendriks, A. W.: Purification of plant nuclei, using colloidal silica. Fed. Europ. Biol. Soc. Letters 24, 101–105 (1972)
Johri, M. M., Varner, J. E.: Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid. Proc. nat. Acad. Sci. (Wash.) 59, 269–276 (1968)
Kuehl, L.: Isolation of plant nuclei. Z. Naturforsch. 19b, 525–532 (1964)
Lieb, H. B., Ray, T. B., Still, C. C.: Growth of rice root-derived callus tissue in suspension culture. Plant Physiol. 51, 1140–1141 (1973)
Rho, J. H., Chipchase, M. I.: Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei. J. Cell Biol. 14, 183–192 (1962)
Tautvydas, K. J.: Mass isolation of pea nuclei. Plant Physiol. 47, 499–503 (1971)
About this article
Cite this article
App, A., Granados, R.R. Isolation of nuclei from rice tissue grown in suspension culture. Planta 120, 201–205 (1974). https://doi.org/10.1007/BF00384931
- Suspension Culture
- Oryza Sativa
- Oriented Research
- Rice Tissue