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Isolation of nuclei from rice tissue grown in suspension culture

Summary

A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.

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App, A., Granados, R.R. Isolation of nuclei from rice tissue grown in suspension culture. Planta 120, 201–205 (1974). https://doi.org/10.1007/BF00384931

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Keywords

  • Cellulase
  • Suspension Culture
  • Oryza Sativa
  • Oriented Research
  • Rice Tissue