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Isolation of nuclei from rice tissue grown in suspension culture


A simple method is described for the isolation of intact nucleic from rice tissue (Oryza sativa L.) grown in suspension culture. The procedure involves incubation of the tissue for 4 h with cellulase and pectinase prior to disruption of the cells. The yield of nucleic is approximately 40% (DNA basis) and the preparations are capable of synthesizing RNA in vitro. The method may be valuable to biochemically oriented research requiring plant nuclei.

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  1. D'Alessio, G., Trim, A. R.: A method for the isolation of nucleic from leaves. J. exp. Bot. 19, 831–839 (1968)

  2. Edmonds, M.: A cytidine triphosphate polymerase from thymus nuclei. J. biol. Chem. 240, 4621–4628 (1965)

  3. Giles, K. W., Myers, A.: An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature (Lond.) 206, 93 (1965)

  4. Hamilton, R. H., Künsch, U., Temperli, A.: Simple rapid procedures for isolation of tobacco leaf nuclei. Analyt. Biochem. 49, 48–57 (1972)

  5. Hartmann, J. X., Kao, K. N., Gamborg, O. L., Miller, R. A.: Immunological methods for the agglutination of protoplasts from cell suspension cultures of different genera. Planta (Berl.) 112, 45–56 (1973)

  6. Hendriks, A. W.: Purification of plant nuclei, using colloidal silica. Fed. Europ. Biol. Soc. Letters 24, 101–105 (1972)

  7. Johri, M. M., Varner, J. E.: Enhancement of RNA synthesis in isolated pea nuclei by gibberellic acid. Proc. nat. Acad. Sci. (Wash.) 59, 269–276 (1968)

  8. Kuehl, L.: Isolation of plant nuclei. Z. Naturforsch. 19b, 525–532 (1964)

  9. Lieb, H. B., Ray, T. B., Still, C. C.: Growth of rice root-derived callus tissue in suspension culture. Plant Physiol. 51, 1140–1141 (1973)

  10. Rho, J. H., Chipchase, M. I.: Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei. J. Cell Biol. 14, 183–192 (1962)

  11. Tautvydas, K. J.: Mass isolation of pea nuclei. Plant Physiol. 47, 499–503 (1971)

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App, A., Granados, R.R. Isolation of nuclei from rice tissue grown in suspension culture. Planta 120, 201–205 (1974).

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  • Cellulase
  • Suspension Culture
  • Oryza Sativa
  • Oriented Research
  • Rice Tissue