Planta

, Volume 120, Issue 2, pp 125–134 | Cite as

Changes in specific radioactivities of sunflower leaf metabolites during photosynthesis in 14CO2 and 12CO2 at normal and low oxygen

  • J. D. Mahon
  • H. Fock
  • D. T. Canvin
Article
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Summary

Sunflower (Helianthus annuus L.) leaf discs were exposed to 14CO2 or 14CO2 followed by 12CO2 in an open gas-exchange system with incoming gas of approximately 400 ppm CO2 and either 21% or 1% O2. The 14CO2 and 12CO2 gas-exchange of the leaf discs were measured, and the specific activities of several metabolites were determined after different lengths of time. The rate of CO2 efflux by the leaf discs was ca. 20% of the net photosynthetic rate at 21% O2 but no CO2 efflux could be detected at 1% O2. At both O2 concentrations the specific activity of 3-phosphoglyceric acid (3-PGA) increased and decreased rapidly for the first 5 min, and then more slowly during 14CO2 feeding and 12CO2 flushing. At 21% O2, glycine, serine and alanine changed more slowly in specific activity than 3-PGA and at 1% O2 their specific activities were much lower than at 21% O2. The results at both O2 concentrations indicated that the glycolate pathway compounds were not derived solely from Calvin-cycle intermediates. At 1% O2 the flux of carbon from the immediate fixation products was inhibited and serine was at least partially produced from a precursor of higher specific activity than glycine, although the glycolate pathway may have been active even at 1% O2. The difference between the specific activities of 3-PGA and the feeding gases could be explained by the recycling of C from the glycolate pathway.

Keywords

Leaf Disc Flushing Specific Radioactivity Helianthus Annuus Fixation Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

3-PGA

3-phosphoglyceric acid

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References

  1. Andrews, T. J., Lorimer, G. H., Tolbert, N. E.: Ribulose diphosphate oxygenase. I. Synthesis of phosphoglycolate by fraction-1 protein of leaves. Biochemistry 12, 11–18 (1973)Google Scholar
  2. Atkins, C. A., Canvin, D. T.: Photosynthesis and CO2 evolution by laaf discs: gas exchange, extraction and ion-exchange fractionation of 14C-labeled photosynthetic products. Canad. J. Bot. 49, 1225–1234 (1971)Google Scholar
  3. Bird, I. F., Cornelius, M. J., Keys, A. J., Whittingham, C. P.: Oxidation and phosphorylation associated with the conversion of glycine to serine. Phytochemistry 11, 1587–1594 (1972)Google Scholar
  4. Bowes, G., Ogren, W. L.: Oxygen inhibition and other properties of soybean ribulose 1,5-diphosphate carboxylase. J. biol. Chem. 247, 2171–2176 (1972)Google Scholar
  5. D'Aoust, A. L., Canvin, D. T.: The specific activity of 14CO2 evolved into CO2-free air in the light and darkness by sunflower leaves following periods of photosynthesis in 14CO2. Photosynthetica 6, 150–157 (1972)Google Scholar
  6. Fock, H., Becker, J. D., Egle, K.: Use of labeled carbon dioxide for separation of CO2 evolution from true CO2 uptake by photosynthesizing Amaranthus and sunflower leaves. Canad. J. Bot. 48, 1185–1189 (1970)Google Scholar
  7. Fock, H., Egle, K.: Über die Beziehungen zwischen dem Glykolsäure-Gehalt und dem Photosynthese-Gaswechsel von Bohnenblättern. Z. Pflanzenphysiol. 57, 389–397 (1967)Google Scholar
  8. Fock, H., Höhler, T., Canvin, D. T., Grant, B. R.: Intermediates of photosynthesis in relation to CO2 evolution in the light. In: Proc. II. Internat. Cgr. on photosynthesis Res. p. 1883–1892, G. Forti, M. Avron, A. Melandri, eds. The Hague: Junk 1972Google Scholar
  9. Galmiche, J. E.: Studies on the mechanism of glycerate 3-phosphate synthesis in tomato and maize leaves. Plant Physiol. 51, 512–519 (1973)Google Scholar
  10. Hoagland, D. R., Arnon, D. J.: The water culture method for growing plants without soil. Univ. Calif. Coll. Agric. Circ. 347, 1–39 (1938)Google Scholar
  11. Jackson, W. A., Volk, R. J.: Photorespiration. Ann. Rev. Plant Physiol. 21, 385–432 (1970)CrossRefGoogle Scholar
  12. Kisaki, T., Yano, N., Hirabayashi, S.: Photorespiration: stimulation of glycine decarboxylation by oxygen in tobacco leaf discs and corn leaf segments. Plant and Cell Physiol. 13, 581–584 (1972)Google Scholar
  13. Latzko, E., Gibbs, M.: Measurement of the intermediates of the photosynthetic carbon reduction cycle, using enzymatic methods. In: Methods in Enzymology, vol. XXIV A, p. 261–268, A. San Pietro, ed. New York: Acad. Press 1972Google Scholar
  14. Ludwig, L. J., Canvin, D. T.: An open gas-exchange system for the simultaneous measurement of the CO2 and 14CO2 fluxes from leaves. Canad. J. Bot. 49, 1299–1313 (1971a)Google Scholar
  15. Ludwig, L. J., Canvin, D. T.: The rate of photorespiration during photosynthesis and the relationship of the substrate of light respiration to the products of photosynthesis in sunflower leaves. Plant Physiol. 48, 712–719 (1971b)Google Scholar
  16. Mahon, J. D., Fock, H., Canvin, D. T.: Changes in specific radioactivities of sunflower leaf metabolites during photosynthesis in 14CO2 and 12CO2 at three concentrations of CO2. Planta (Berl.) in press (1974b)Google Scholar
  17. Mahon, J. D., Fock, H., Höhler, T., Canvin, D. T.: Changes in specific radioactivities of corn leaf metabolites during photosynthesis in 14CO2 and 12CO2 at normal and low oxygen. Planta (Berl.) in press (1974a)Google Scholar
  18. Osmond, C. B., Björkman, O.: Simultaneous measurements of oxygen effects on net photosynthesis and glycolate metabolism in C3 and C4 species of Atriplex. Carnegie Instn. YrBk. 1972, 141–148Google Scholar
  19. Roach, D., Gehrke, C. W.: The gas-liquid chromatography of amino acids. J. Chromatography 43, 303–310 (1969a)Google Scholar
  20. Roach, D., Gehrke, C. W.: Direct esterification of the protein amino acids; gas-liquid chromatography of N-TFA n-butyl esters. J. Chromatography 44, 269–278 (1969b)Google Scholar
  21. Samish, J., Pallas, J. E., Dornhoff, G. M., Shibles, R. M.: A re-evaluation of soybean leaf photorespiration. Plant Physiol. 50, 28–30 (1972)Google Scholar
  22. Shain, Y., Gibbs, M.: Formation of glycolate by a reconstituted spinach chloroplast preparation. Plant Physiol. 48, 325–330 (1971)Google Scholar
  23. Tolbert, N. E.: Leaf peroxisomes and photorespiration. In: Photosynthesis and photorespiration. p. 458–471, M. D. Hatch, C. B. Osmond, R. O. Slatyer, eds. New York-London: Wiley-Interscience 1971Google Scholar
  24. Voskresenskaya, N. P., Wiil, Y. A., Grishina, G. S., Pärnik, T. R.: Effect of oxygen concentration and light intensity on the distribution of labelled carbon in photosynthesis products in bean plants. Photosynthetica 4, 1–8 (1970)Google Scholar
  25. Zelitch, I.: Photosynthesis photorespiration and plant productivity. New York-London: Acad. Press 1971Google Scholar
  26. Zelitch, I.: Comparison of the effectiveness of glycolic acid and glycine as substrates for photorespiration. Plant Physiol. 50, 109–113 (1972)Google Scholar
  27. Zelitch, I.: Alternate pathways of glycolate synthesis in tobacco and maize leaves in relation to rates of photorespiration. Plant Physiol. 51, 299–305 (1973)Google Scholar
  28. Zumwalt, R. W., Roach, D., Gehrke, C. W.: Gas-liquid chromatography of amino acids in biological substances. J. Chromagraphy 53, 171–194 (1970)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • J. D. Mahon
    • 1
  • H. Fock
    • 2
  • D. T. Canvin
    • 3
  1. 1.Fachbereich Biologie der UniversitätFrankfurt a.M.Federal Republic of Germany
  2. 2.Fachbereich Biologie der UniversitätKaiserslauternFederal Republic of Germany
  3. 3.Department of BiologyQueen's UniversityKingstonCanada

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