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Photosynthetic apparatus in chilling-sensitive plants

II. Changes in free fatty acid composition and photoperoxidation in chloroplasts following cold storage and illumination of leaves in relation to Hill reaction activity

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Abstract

The composition of free fatty acids (FFA) in relation to Hill reaction activity and photoperoxidation of lipids was studied in chloroplasts isolated from fresh, cold and dark-stored as well as illuminated leaves of Lycopersicon esculentum Mill., Phaseolus vulgaris L. and Cucumis sativus L. Following the cold and dark-storage of leaves the loss of Hill reaction activity is accompanied by approximately a 5-fold increase in the amount of FFA and by an increase in the percentage of unsaturated FFA, particularly that of linolenic acid. Illumination of the cold- and dark-stored leaves restores both Hill reaction activity and the content and composition of chloroplast FFA. Following the second and third cycles of cold storage and illumination of leaves the percentage of unsaturated fatty acids in chloroplasts increases while that of saturated ones decreases despite of the significant restoration of Hill reaction activity. Since the illumination of cold-stored leaves results in peroxidation of inhibitory fatty acids it seems likely that this phenomenon could, at least partially, be responsible for the restoration of Hill reaction activity. Inhibition of Hill reaction activity by exogenous linolenic acid in chloroplasts of fresh, cold-stored as well as cold-stored and illuminated leaves could be reversed following the incubation of chloroplast suspension with BSA, however only to a value measured in the absence of unsaturated fatty acid. All these results indicate that the inhibition of Hill reaction activity due to the cold and dark storage of leaves is caused by both inhibitory FFA released from chloroplast lipids as well as by damage to the thylakoid structure affecting the electron transport within photosystem II.

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Abbreviations

BSA:

bovine serum albumin

DCIP:

2,6-dichlorophenolindophenol

DGDG:

digalactosyl diglyceride

HEPES:

2-(4(2-hydroxyethyl)-piperazinyl) ethanesulfonic acid

FFA:

free fatty acids

MDA:

malondialdehyde

MGDG:

monogalactosyldiglyceride

TBA:

thiobarbituric acid

Tris:

tris-(Hydroxymethyl)aminomethane

References

  1. Anderson, M.M., McCarty, R.E.: Rapid and sensitive assay for free fatty acids using Rhodamine 6G. Anal. Biochem. 45, 260–270 (1972)

  2. Anderson, M.M., McCarty, R.E., Zimmer, E.A.: The role of galactolipids in spinach chloroplast lamellar membranes. I. Partial purification of bean leaf galactolipid lipase and its action on subchloroplast particles. Plant Physical. 53, 699–704 (1974)

  3. Chen, R.F.: Removal of fatty acids from serum albumin by charcoal treatment. J. biol. Chem. 242, 173–181 (1967)

  4. Constantopoulos, G., Kenyon, C.N.: Release of free fatty acids and loss of Hill activity by aging spinach chloroplasts. Plant Physiol. 43, 531–536 (1968)

  5. De Kok, L.J., Kuiper, P.J.C.: Glycolipid degradation in leaves of the thermophilic Cucumis sativus as affected by light and low-temperature treatment. Physiol. Plant. 39, 123–128 (1977)

  6. Folch, J., Lees, M., Stanley, S.: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957)

  7. Friedlander, M., Neumann, L.: Stimulation of photoreactions of isolated chloroplasts by serum albumin. Plant Physiol. 43, 1249–1254 (1968)

  8. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 189–198 (1968a)

  9. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. II. Role of electron transfer. Arch. Biochem. Biophys. 125, 850–857 (1968b)

  10. Helmsing, P.J.: Hydrolysis of galctolipids by enzymes in spinach leaves. Biochim. Biophys. Acta 144, 470–472 (1967)

  11. Hoshina, S., Kaji, F., Nishida, K.: Photoswelling and lightinactivation of isolated chloroplasts. I. Changes in lipid content in light-aged chloroplast. Plant & Cell Physiol. 16, 465–474 (1975)

  12. Jamieson, G.R., Reid, E.H.: The occurrnce of hexadeca-7, 10, 13-trienoic acid in the leaf lipids of angiosperms. Phytochemistry 10, 1837–1843 (1971)

  13. Kaninga, Z., Frąckowiak, B., Michalski, W.: Role of fatty acids and Mn++ in the restoration of Hill activity in chloroplasts of chilling-sensitive plants following cold-storage. In: Abstr. 10th Meet. Fed. Eur. Biochem. Soc. No 1121, Paris 1975

  14. Kaniuga, Z., Sochanowicz, B., Ząbek, J., Krzystyniak, K.: Photosynthetic apparatus in chilling-sensitive plants. I. Reactivation of Hill reaction activity inhibited on the cold and dark-storage of detached leaves and intact plants. Planta in press (1978)

  15. Kates, M.: Plant phospholipids and glycolipids. Adv. Lipid Res. 8, 225–265 (1970)

  16. Kulandaivelu, G., Hall, D.O.: Stabilization of photosynthetic activities of isolated spinach chloroplasts during prolonged ageing. Z. Naturforsch. 31c, 452–455 (1976)

  17. Kwon, T., Menzel, D.B., Olscott, H.S.: Reactivity of malonaldehyde with food constituents. J. Food Sci. 30, 808–813 (1965)

  18. Lyons, J.M.: Chilling injury in plants. Ann. Rev. Plant Physiol. 24, 445–466 (1973)

  19. Mazliak, O.P.: Lipid metabolism in plants. Ann. Rev. Plant Physiol. 24, 287–310 (1973)

  20. Radin, N.S.: Preparation of lipid extracts. In: Methods in enzymology, Vol. XIV, Lipds, pp. 245–254, Loevenstein, J.M. ed. New York: Academic Press 1969

  21. Raison, J.K.: Temperature-induced phase changes in membrane lipids and their influence on metabolic regulation. Symp. Soc. exp. Biol. 27, 485–512 (1973)

  22. Sastry, P.S., Kates, M.: Hydrolysis of monogalactosyl and digalactosyl diglycerides by specific enzymes in runner-bean leaves. Biochemistry 3, 1280–1287 (1964)

  23. Schwertner, H.A., Biale, J.B.: Lipid composition of plant mitochondria and chloroplasts. J. Lipid Res. 14, 235–242 (1973)

  24. Siegenthaler, P.A.: Aging of the photosynthetic apparatus. IV. Similarity between the effects of aging and unsaturated fatty acids on isolated spinach chloroplasts as expressed by volume changes. Biochim. Biophys. Acta 275, 182–191 (1972)

  25. Smoleńska, G., Kuiper, P.J.C.: Effect of low temperature upon lipid and fatty acid composition of roots and leaves of winter rape plants. Physiol. Plant. 41, 29–35 (1977)

  26. Takahama, U., Nishimura, M.: Formation of singlet molecular oxygen in illuminated chloroplasts: Effects on photoinactivation and lipid peroxidation. Plant & Cell Physiol. 16, 737–748 (1975)

  27. Takahama, U., Nishimura, M.: Effects of electron donors and acceptors, electron transfer mediators and superoxide dismutase on lipid peroxidation in illuminated chloroplasts fragments. Plant & Cell Physiol. 17, 111–118 (1976)

  28. van Hasselt, Ph.R.: Photooxidation of unsaturated lipids in Cucumis leaf discs during chilling. Acta Bot. Neerl. 23, 159–169 (1974)

  29. Wasserman, A.R., Fleisher, S.: The stabilization of chloroplast function. Biochim. Biophys. Acta 153, 154–169 (1968)

  30. Wilson, J.M., Crawford, R.M.M.: Leaf fatty-acid content in relation to hardening and chilling-injury. J. exp. Bot. 25, 121–131 (1974)

  31. Wintermans, J.F.G.M., Helmsing, P.J., Polman, B.J.J., van Gisbergen, J., Collard, J.: Galactolipid transformations and photochemical activities of spinach chloroplasts. Biochim. Biophys. Acta 189, 95–105 (1969)

  32. Yamashita, T., Butler, W.L.: Inhibition of the Hill reaction by Tris and restoration by electron donation to photosystem II. Plant Physiol. 44, 435–438 (1969)

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Kaniuga, Z., Michalski, W. Photosynthetic apparatus in chilling-sensitive plants. Planta 140, 129–136 (1978). https://doi.org/10.1007/BF00384911

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Key words

  • Chilling
  • Chloroplasts
  • Cold storage
  • Cucumis
  • Lycopersicon
  • Phaseolus
  • Photosynthesis
  • Temperature (chilling)