Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The control of glycolysis in aged slices of carrot root tissue


The possibility that the rate of glycolysis in aged slices of carrot (Daucus carota L.) is controlled by the enzyme phosphofructokinase was examined, by changing the rate of metabolism, by supplying the tissue with potassium chloride, potassium phosphate and potassium citrate and measuring the subsequent changes in levels of metabolites. Potassium chloride and potassium phosphate stimulate glycolysis, potassium citrate inhibits glycolysis and the associated changes in metabolites are consistent with the view that respiration is controlled by a dual system involving phosphofructokinase and glyceraldehyde phosphate dehydrogenase or possibly phosphoglycerate kinase. It is proposed that the control points are interlocked by phosphoenolpyruvate and phosphoglycerate. Thus if glyceraldehyde phosphate dehydrogenase is activated leading to an accumulation of phosphoglycerate and phosphoenolpyruvate, these compounds will inhibit phosphofructokinase. Thus our proposal for metabolic control in carrot resembles those proposed in mammalian systems except that the negative feedback system involving ATP and AMP which controls phosphofructokinase in mammals is replaced by a negative feedback system involving phosphoenolpyruvate and phosphoglycerate.

This is a preview of subscription content, log in to check access.


  1. Adams, P. B., Rowan, K. S.: Glycolytic control of respiration during ageing of carrot root tissue. Plant Physiol. 45, 500–503 (1970)

  2. Adams, P. B., Rowan, K. S.: Regulation of salt respiration in carrot root slices. Plant Physiol. 50, 682–686 (1972)

  3. Ap Rees, T., Blanch, E., Graham, D., Davies, D. D.: Recycling in the pentose phosphate Pathway comparison of C6/C1 ratios measured with glucose-14C and fructose-14C. Plant Physiol. 40, 910–914 (1965)

  4. Barker, J., Khan, M. A. A., Solomos, T.: Studies in the respiratory and carbohydrate metabolism of plant tissues. XXI. The mechanism of the Pasteur effect in peas. New Phytologist 66, 577–596 (1967)

  5. Bennet-Clark, T. A., Bexon, D.: Water relations of plant cells. III. The respiration of plasmolysed tissues. New Phytologist 42, 65–92 (1943)

  6. Dennis, D. T., Coultate, T. P.: Phosphofructokinase, a regulatory enzyme in plants. Biochem. biophys. Res. Commun. 25, 187–191 (1966)

  7. Dennis, D. T., Coultate, T. P.: The regulatory properties of a plant phosphofructokinase during leaf development. Biochim. biophys. Acta (Amst.) 146, 129–137 (1967)

  8. Faiz-ur-Rahman, A. T. M., Trewavas, A. J., Davies, D. D.: The Pasteur effect in carrot root tissue. Planta (Berl.) 118, 195–210 (1974)

  9. Fowler, M. W.: Studies on the growth in culture of plant cells. XIV. Carbohydrate oxidation during the growth of Acer pseudoplatanus L. cells in suspension culture. J. exp. Bot. 22, 715–724 (1971)

  10. Garrard, L. A., Humphreys, T. E.: Aerobic fermentation and phosphofructokinase in tissue slices of the Corn scutellum. Phytochemistry 7, 1949–1961 (1968)

  11. Givan, C. V.: Short-term changes in hexose phosphates and ATP in intact cells of Acer pseudoplatanus L. Plant Physiol. 43, 948–952 (1968)

  12. Givan, C. V.: Control of glycolysis by orthophosphate and adenosine triphosphate m soluble extracts of germinating Pea seeds. Planta (Berl.) 108, 29–38 (1972)

  13. Kelly, G. J., Turner, J. F.: Inhibition of pea-seed phosphofructokinase by phosphenolpyruvate. Biochem. biophys. Res. Commun. 30, 195–199 (1960)

  14. Kelly, G. J., Turner, J. F.: The regulation of Pea-seed phosphofructokinase by phosphoenolpyruvate. Biochem. J. 115, 481–487 (1969)

  15. Krebs, H. A.: Gluconeogenesis. Proc. roy. Soc. B 159, 545–564 (1964)

  16. Krebs, H. A.: The Pasteur effect and the relations between respiration and fermentation. Essays Biochem. 9, 1–34 (1973)

  17. Newsholme, E. A.: Theoretical and experimental considerations on the control of glycolysis in muscle. In: Essays in cell metabolism, p. 189–223, eds. Bartley, W., Kornberg, H. L., Quayle, J.. London: Wiley-Interscience 1970

  18. Newsholme, E. A., Start, C.: Regulation in metabolism. New York: J. Wiley & Sons 1973

  19. Passonneau, J. V., Lowry, O. H.: The role of phosphofructokinase in metabolic regulation. Adv. Enz. Regul. G. 2, 265–274 (1964)

  20. Robertson, R. N.: Protons, electrons, phosphorylation and active transport. Cambridge: Univ. Press 1968

  21. Sasaki, T., Tadokoro, K., Suzuki, S.: Phosphofructokinase of Solanum tuberosum Tuber. Phytochemistry 12, 2843–2849 (1973)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Faiz-ur-Rahman, A.T.M., Davies, D.D. & Trewavas, A.J. The control of glycolysis in aged slices of carrot root tissue. Planta 118, 211–224 (1974).

Download citation


  • Respiration
  • Control Point
  • Root Tissue
  • Metabolic Control
  • Glyceraldehyde Phosphate Dehydrogenase