Advertisement

Planta

, Volume 108, Issue 3, pp 283–287 | Cite as

Failure of colchicine or cytochalasin to inhibit protein secretion by plant cells

  • Maarten J. Chrispeels
Short Communication

Summary

The gibberellic-acid-mediated secretion of α-amylase by aleurone cells of barley and the transport of hydroxyproline-rich cell-wall glycoproteins from the cytoplasm to the wall in phloem-parenchyma cells of carrot roots are not inhibited by either colchicine (1 mM) or cytochalasin (10 μg/ml). The data suggest the absence of a unitary mechanism of protein secretion.

Keywords

Plant Cell Colchicine Protein Secretion Cytochalasin Aleurone Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adelman, M. R., Borisy, G. G., Shelanski, M. L., Weisenberg, R. C., Taylor, E. W.: Cytoplasmie filaments and tubules. Fed. Proc. 27, 1186–1193 (1968).Google Scholar
  2. 2.
    Amsterdam, A., Ohad, L., Schramm, M.: Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. J. Cell Biol. 41, 753–773 (1969).CrossRefPubMedGoogle Scholar
  3. 3.
    Chrispeels, M. J.: Synthesis and secretion of hydroxyproline containing macromolecules in carrots. I. Plant Physiol. 44, 1187–1193 (1969).Google Scholar
  4. 4.
    Chrispeels, M. J., Varner, J. E.: Gibberellic acid-enchanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406 (1967).Google Scholar
  5. 5.
    Diegelmann, R. F., Peterkofsky, B.: Inhibition of collagen secretion from bone and cultured fibroblasts by microtubular disruptive drugs. Proc. nat. Acad. Sci. (Wash.) 69, 892–896 (1972).Google Scholar
  6. 6.
    Doerschug, M. R., Chrispeels, M. J.: Synthesis and secretion of hydroxyproline containing macromolecules in carrots. III. Plant Physiol. 46, 363–366 (1970).Google Scholar
  7. 7.
    Jamieson, J. D., Palade, G. E.: Role of the Golgi complex in the intracellular transport of secretory proteins. Proc. nat. Acad. Sci. (Wash.) 55, 424–431 (1966).Google Scholar
  8. 8.
    Jones, R. F.: Fractionation of the enzymes of the barley aleurone layer: evidence for a soluble mode of enzyme release. Planta (Berl.), 103, 95–109 (1972).Google Scholar
  9. 9.
    Lacy, P. E., Howell, S. L., Young, O. A., Fink, C. J.: New hypothesis of insulin secretion. Nature (Lond.) 219, 1177–1179 (1968).Google Scholar
  10. 10.
    Lamport, D. T. A.: The protein component of primary cell walls. Advanc. Bot. Res. 2, 151–218 (1965).Google Scholar
  11. 11.
    Mascarenhas, J. P., LaFountain, J.: Protoplasmic streaming, cytochalasin B and growth of the pollen tube. Tissue and Cell 4, 11–14 (1972).PubMedGoogle Scholar
  12. 12.
    Parkhouse, R. M. E., Allison, A. C.: Failure of cytochalasin or colchicine to inhibit secretion of immunoglobulins. Nature (Lond.) New Biology 235, 220–222 (1972).Google Scholar
  13. 13.
    Pickett-Heaps, J. D.: The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation and distribution of cytoplasmic microtubules. Develop. Biol. 15, 206–236 (1967).Google Scholar
  14. 14.
    Rosen, W. G., Gawlik, S. R., Dashek, W. V., Seigesmund, K. A.: Fine structure and cytochemistry of Lilium pollen tubes. Amer. J. Bot. 51, 61–71 (1964).Google Scholar
  15. 15.
    Rosenbloom, J., Prockop, D. J.: Biochemical aspects of collagen biosynthesis. In: Repair and regeneration: scientific basis for medical practice, p. 117–135, J. E. Dunphy, W. Van Winkle, eds. New York: McGraw-Hill 1968.Google Scholar
  16. 16.
    Schofield, J. G.: Cytochalasin B and release of growth hormone. Nature (Lond.) New Biol. 234, 215–216 (1971).Google Scholar
  17. 17.
    Wessels, N. K., Spooner, B. S., Ash, J. F., Bradley, M. O., Luduenda, M. A., Taylor, F. L., Wrenn, J. T., Yamada, K. M.: Microfilaments in cellular and developmental processes. Science 171, 135–143 (1971).PubMedGoogle Scholar
  18. 18.
    Williams, J. A., Wolff, J.: Possible role for microtubules in thyroid secretion. Proc. nat. Acad. Sci. (Wash.) 67, 1901–1908 (1970).Google Scholar
  19. 19.
    Zagury, D., Uhr, J. W., Jamieson, J. D., Palade, G. E.: Immunoglobulin synthesis and secretion II. J. Cell Biol. 46, 52–63 (1970).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Maarten J. Chrispeels
    • 1
  1. 1.Department of Biology, John Muir CollegeUniversity of California/San DiegoLa JollaUSA

Personalised recommendations