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Planta

, Volume 123, Issue 2, pp 175–184 | Cite as

Photosynthesis and the induction of nitrate reductase and nitrite reductase in bean leaves

  • C. M. Th. Sluiters-Scholten
Article

Summary

In laaves of Phaseolus vulgaris L. cv. Prelude, the light-induced increase in activity of NADH-nitrate oxidoreductase (E.C.1.6.6.2; NAR) and reduced benzylviologennitrite oxidoreductase (E.C.1.6.6.4; NIR) starts at a certain stage in the development of the chloroplasts. In leaves with completely developed chloroplasts, a higher increase in activity of NAR and NIR is observed, after induction by the addition of nitrate, in the light than in the dark. DCMU inhibits the increase in activity of the two enzymes in the light. Both in the light in the presence of DCMU, and in the dark the increase in activity reaches a higher level by the addition of sucrose.

Induction of NAR, but not of NIR, can be observed in excised etiolated leaves. No induction is found in leaves of intact etiolated seedlings.

The relation between photosynthetic reactions and the increase in activity of NAR and NIR is discussed. It is suggested that NADH, indirectly formed by photosynthesis, protects NAR and affects in this way the balance between synthesis and breakdown of the enzyme. The increase in activity of NIR is possibly influenced by the presence of reduced ferredoxin.

Keywords

Enzyme Nitrate Sucrose Nitrite Photosynthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CAP

D-threo-chloramphenicol

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

NAR

nitrate reductase

NIR

nitrite reductase

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • C. M. Th. Sluiters-Scholten
    • 1
  1. 1.Department of Plant PhysiologyUniversity of AmsterdamAmsterdamThe Netherlands

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