Molecular and General Genetics MGG

, Volume 194, Issue 1–2, pp 303–309 | Cite as

Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12

  • Robert G. Lloyd
  • Fiona E. Benson
  • Claire E. Shurvinton


Mutation of the ruv gene of E. coli is associated with sensitivity to radiation, and filamentous growth after transient inhibition of DNA synthesis. The filamentation of ruv strains is abolished by mutations in sfiA or sfiB that prevent SOS induced inhibition of cell division, but this does not restore resistance to UV radiation. Double mutants carrying both ruv and uvr mutations are considerably more sensitive to UV radiation than the single mutants, but there is no additive effect of ruv with recA, recF, recB, or recC mutations. ruv mutations have little effect on conjugal recombination in wild-type strains but confer recombination deficiency and extreme sensitivity to ionizing radiation in recBC sbcB strain. These results, together with the fact that ruv strains are excision proficient and mutable by UV light, are interpreted to suggest that the ruv+ product is involved in recombinational repair of damaged DNA rather than in cell division as suggested by Otsuji et al. (1974).


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Armengod M (1982) recF-dependent recombination as a SOS function. Biochimie 64:629–632PubMedGoogle Scholar
  2. Bachmann BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36:525–557PubMedGoogle Scholar
  3. Berman ML, Enquist LW, Silhavy TJ (1981) Advanced bacterial genetics. Cold Sring Harbor Laboratory, New YorkGoogle Scholar
  4. Bresler SE, Goryshin IYu, Lanzov VA (1981) The process of general recombination in Escherichia coli K-12. Structure of intermediate products. Mol Gen Genet 183:139–143CrossRefPubMedGoogle Scholar
  5. Ganesan AK, Seawell PC (1975) The effect of lexA and recF mutations on postreplication repair and DNA synthesis in Escherichia coli K-12. Mol Gen Genet 141:189–205PubMedGoogle Scholar
  6. George J, Castellazzi M, Buttin G (1975) Prophage induction and cell division in E. coli III. Mutations sfiA and sfiB restore division in tif and lon strains and permit the expression of mutator properties of tif. Mol Gen Genet 140:309–332PubMedGoogle Scholar
  7. Hall JD, Mount DW (1981) Mechanisms of DNA replication and mutagenesis in ultraviolet-irradiated bacteria and mammlian cells. Prog Nucl Acid Res Mol Biol 25:54–126Google Scholar
  8. Horii ZI, Clark AJ (1973) Genetic analysis of the RecF pathway to genetic recombination in Escherichia coli K-12: isolation and characterization of mutants. J Mol Biol 80:327–344PubMedGoogle Scholar
  9. Howard-Flanders P, Simson E, Theriot L (1964) A locus that controls filament formation and sensitivity to radiation in Escherichia coli K-12. Genetics 49:237–246PubMedGoogle Scholar
  10. Huisman O, D'Ari R (1981) An inducible DNA replication-cell division coupling mechanism in E. coli. Nature 290:797–799PubMedGoogle Scholar
  11. Huisman O, D'Ari R, George J (1980) Further characterization of sfiA and sfiB mutations in Escherichia coli. J Bacteriol 144:185–191PubMedGoogle Scholar
  12. Iyehara H, Otsuji N (1975) Ultraviolet light sensitivity of strains of Escherichia coli K-12 carrying ruv mutations in combination with uvrA or lon mutant alleles. J Bacteriol 121:735–736PubMedGoogle Scholar
  13. Krasin F, Hutchinson F (1981) Repair of DNA double strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light. Proc Natl Acad Sci USA 78:3450–3453PubMedGoogle Scholar
  14. Kushner SR, Nagaishi H, Templin A, Clark AJ (1971) Genetic recombination in Escherichia coli: the role of exonuclease I. Proc Natl Acad Sci USA 67:824–827Google Scholar
  15. Little JW, Mount DW (1982) The SOS regulatory system of Escherichia coli. Cell 29:11–22CrossRefPubMedGoogle Scholar
  16. Livneh Z, Lehman IR (1982) Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli. Proc Natl Acad Sci USA 79:3171–3175PubMedGoogle Scholar
  17. Lloyd RG (1983) lexA dependent recombination in uvrD strains of Escherichia coli. Mol Gen Genet 189:157–161PubMedGoogle Scholar
  18. Lloyd RG, Barbour SD (1974) The genetic location of the sbcA gene of Escherichia coli. Mol Gen Genet 134:157–171PubMedGoogle Scholar
  19. Lloyd RG, Johnson S (1979) Kinetics of conjugational recombinant formation. Mol Gen Genet 169:219–228CrossRefGoogle Scholar
  20. Lloyd RG, Low B (1976) Some genetic consequences of changes in the level of recA gene function in Escherichia coli K-12. Genetics 84:675–695PubMedGoogle Scholar
  21. Lloyd RG, Thomas A (1983) On the nature of the RecBC and RecF pathways of conjugal recombination in Escherichia coli. Mol Gen Genet 190:156–161PubMedGoogle Scholar
  22. Lloyd RG, Low B, Godson GN, Birge EA (1974) Isolation and characterisation of an Escherichia coli K-12 mutant with a temperature-sensitive RecA phenotype. J Bacteriol 120:407–415PubMedGoogle Scholar
  23. Lloyd RG, Picksley SM, Prescott C (1983) Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K-12. Mol Gen Genet 190:162–167PubMedGoogle Scholar
  24. Lovett ST, Clark AJ (1983) Genetic analysis of the RecF pathway of recombination in Escherichia coli K-12. J Bacteriol 153:1471–1478PubMedGoogle Scholar
  25. Mahajan SK, Datta AR (1979) Mechanisms of recombination by the RecBC and the RecF pathways following conjugation in Escherichia coli K-12. Mol Gen Genet 169:67–78PubMedGoogle Scholar
  26. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  27. Mizusawa S, Gottesman S (1983) Protein degradation in E. coli. The lon gene controls the stability of sulA protein. Proc Natl Acad Sci USA 80:358–362PubMedGoogle Scholar
  28. Otsuji N, Iyehara-Ogawa H (1979) Thermoresistant revertants of an Escherichia coli strain carrying tif-1 and ruv mutations: Non-suppressibility of ruv by sfi. J Bacteriol 138:1–6PubMedGoogle Scholar
  29. Otsuji N, Iyehara H, Hideshima Y (1974) Isolation and characterisation of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation. J Bacteriol 117:337–344PubMedGoogle Scholar
  30. Pang PP, Walker GC (1983) The Salmonella typhimurium LT2 uvrD gene is regulated by the lexA gene product. J Bacteriol 154:1502–1504PubMedGoogle Scholar
  31. Ream LW, Margossian L, Clark AJ, Hansen FG, von Meyenberg K (1980) Genetic and physical mapping of recF in Escherichia coli K-12. Mol Gen Genet 180:115–121PubMedGoogle Scholar
  32. Rothman RH, Clark AJ (1977) The dependence of postreplication repair on uvrB in a recF mutant of Escherichia coli K-12. Mol Gen Genet 155:279–286PubMedGoogle Scholar
  33. Rupp WD, Howard-Flanders P (1968) Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J Mol 31:291–304Google Scholar
  34. Rupp WD, Wilde CE, Reno DL, Howard-Flanders P (1971) Echanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol 61:25–44PubMedGoogle Scholar
  35. Sancar A, Rupp WD (1983) A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell 33:249–260PubMedGoogle Scholar
  36. Shurvinton CE, Lloyd RG (1982) Damage to DNA induces expression of the ruv gene of Escherichia coli. Mol Gen Genet 185:352–355PubMedGoogle Scholar
  37. Shurvinton CE, Lloyd RG, Benson FE, Attfield PV (1984) Genetic analysis and molecular cloning of the Escherichia coli ruv gene. Mol Gen Genet, in pressGoogle Scholar
  38. Smith KC, Meun DHC (1970) Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation. J Mol Biol 51:459–472PubMedGoogle Scholar
  39. Stacey KA, Lloyd RG (1976) Isolation of Rec mutants from an F-prime merodiploid strain of Escherichia coli K-12. Mol Gen Genet 143:223–232PubMedGoogle Scholar
  40. West SC, Cassuto E, Howard-Flanders P (1982) Postreplication repair in E. coli: Strand exchange reactions of gapped DNA by RecA protein. Mol Gen Genet 187:209–217PubMedGoogle Scholar
  41. Witkin EM (1976) Ultraviolet mutagenesis and inducible deoxyri-bonucleic acid repair in Escherichia coli. Bacteriol Rev 40:869–907PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Robert G. Lloyd
    • 1
  • Fiona E. Benson
    • 1
  • Claire E. Shurvinton
    • 1
  1. 1.Genetics DepartmentUniversity of NottinghamNottinghamEngland

Personalised recommendations