Molecular and General Genetics MGG

, Volume 194, Issue 1–2, pp 65–72 | Cite as

Plasmid RP4 encodes two forms of a DNA primase

  • Erich Lanka
  • Rudi Lurz
  • Manfred Kröger
  • Jens P. Fürste


The pri gene locus of the conjugative broad host range plasmid RP4 maps between coordinates 40.3 and 43.5 and encodes two antigenically related forms of a DNA primase with a molecular mass of 118 and 80 kDa (kilodalton). Genesis of these two products has been examined using Pri+-recombinant plasmids. As shown by deletion analysis, the primase polypeptides are two separate translation products which arise from an in-phase overlapping gene arrangement. It is suggested that transcription of a set of RP4 genes including the pri gene starts at a promoter site within the Tra1 region. In vivo, RP4 mutant primase can apparently substitute for Escherichia coli primase as demonstrated by measuring suppression of the dnaG3(ts) mutant.


Escherichia Coli Molecular Mass Polypeptide Gene Locus Recombinant Plasmid 
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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Erich Lanka
    • 1
  • Rudi Lurz
    • 1
  • Manfred Kröger
    • 2
  • Jens P. Fürste
    • 1
  1. 1.Abt. SchusterMax-Planck-Institut für Molekulare GenetikBerlin 33Federal Republic of Germany
  2. 2.Institut für Biologie IIIUniversität FreiburgFreiburgFederal Republic of Germany

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