Molecular and General Genetics MGG

, Volume 103, Issue 4, pp 305–312 | Cite as

Inactivation of a newly transferred gene in recombination-deficient recipients of Escherichia coli

  • Eugenie Dubnau
  • Werner K. Maas


The expression of a newly transferred lacZ+ gene in lacZ recipients carrying various mutations in the recA and recB genes was studied by measuring the rates of induced synthesis of β-galactosidase in zygotes formed after mating with either F′ or Hfr donors. The ability to synthesize β-galactosidase decreases with time in both recA and recB zygotes when the lacZ+ gene is transferred from an Hfr donor, but not when the lacZ gene is transferred from an F′ donor. There is no such inactivation of the newly transferred lacZ+ gene in Rec+ zygotes. We conclude that the functioning of the transferred DNA is progressively inactivated in rec recipients unless the DNA is contained in an episome such as F′.


Escherichia Coli lacZ Gene 
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Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Eugenie Dubnau
    • 1
  • Werner K. Maas
    • 1
  1. 1.Department of MicrobiologyNew York University Medical SchoolNew York

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