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Molecular and General Genetics MGG

, Volume 103, Issue 4, pp 305–312 | Cite as

Inactivation of a newly transferred gene in recombination-deficient recipients of Escherichia coli

  • Eugenie Dubnau
  • Werner K. Maas
Article

Summary

The expression of a newly transferred lacZ+ gene in lacZ recipients carrying various mutations in the recA and recB genes was studied by measuring the rates of induced synthesis of β-galactosidase in zygotes formed after mating with either F′ or Hfr donors. The ability to synthesize β-galactosidase decreases with time in both recA and recB zygotes when the lacZ+ gene is transferred from an Hfr donor, but not when the lacZ gene is transferred from an F′ donor. There is no such inactivation of the newly transferred lacZ+ gene in Rec+ zygotes. We conclude that the functioning of the transferred DNA is progressively inactivated in rec recipients unless the DNA is contained in an episome such as F′.

Keywords

Escherichia Coli lacZ Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Eugenie Dubnau
    • 1
  • Werner K. Maas
    • 1
  1. 1.Department of MicrobiologyNew York University Medical SchoolNew York

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