Molecular and General Genetics MGG

, Volume 200, Issue 1, pp 65–67

Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria

  • Graham J. Boulnois
  • Jennifer M. Varley
  • Geoffrey S. Sharpe
  • F. Christopher H. Franklin


The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Graham J. Boulnois
    • 1
  • Jennifer M. Varley
    • 1
  • Geoffrey S. Sharpe
    • 2
  • F. Christopher H. Franklin
    • 1
  1. 1.Department of Medical BiochemistryUniversity Medical CentreGeneva 4Switzerland
  2. 2.University/ICI Joint LaboratoryUniversity of LeicesterLeicesterUK
  3. 3.Department of MicrobiologyUniversity of LeicesterLeicesterUK
  4. 4.Department of GeneticsUniversity of BirminghamBirminghamUK

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