Advertisement

Molecular and General Genetics MGG

, Volume 200, Issue 1, pp 33–39 | Cite as

Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3″) (9)

  • Ellen Murphy
Article

Summary

The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G→A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gramnegative streptomycin/spectinomycin adenyltransferase, AAD(3″) (9), specified by the aadA determinant (Holingshead and Vapnek 1985).

Keywords

Spectinomycin Protected Fragment Ribosome Binding Site Sequence LysTyr Benzyloxymethyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alwine JC, Kemp DJ, Parker BA, Reiser J, Renart J, Stark GR, Wahl GM (1979) Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol 68:220–242PubMedCrossRefGoogle Scholar
  2. Benveniste R, Davies J (1973) Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistant bacteria. Proc Natl Acad Sci USA 70:2276–2280PubMedCrossRefGoogle Scholar
  3. Berk AJ, Sharp PA (1977) Sizing and mapping of early adeno-virus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732PubMedCrossRefGoogle Scholar
  4. Biggin MD, Gibson TJ, Hong GF (1983) Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80:3963–3965PubMedCrossRefGoogle Scholar
  5. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299PubMedCrossRefGoogle Scholar
  6. Courvalin P, Carlier C, Collatz E (1981) Evolutionary relationships between plasmid-mediated aminoglycoside-modifying enzymes from gram-positive and gram-negative bacteria. In: Gialdroni-Grassi G, Sabatti L (eds) New trends in antibiotics: Research and therapy. Elsevier/North Holland Biomedical Press, AmsterdamGoogle Scholar
  7. Davies J, Smith DI (1978) Plasmid-determined resistance to antimicrobial agents. Annu Rev Microbiol 32:469–518PubMedCrossRefGoogle Scholar
  8. Drassar FA (1978) Detection of aminoglycoside degrading enzymes. In: Reeves DS, Phillips I, Williams JD, Wise R (eds) Laboratory methods in antimicrobial chemotherapy. Churchill Livingstone, Edinburgh, pp 70–75Google Scholar
  9. Glisin V, Crkvenjakov R, Byus C (1974) Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633–2637PubMedCrossRefGoogle Scholar
  10. Hollingshead S, Vapnek D (1985) Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenyltransferase. Plasmid 13:17–30PubMedCrossRefGoogle Scholar
  11. Iordanescu S (1976) Three distinct plasmids originating in the same Staphylococcus aureus strain. Arch Roum Pathol Exp Microbiol 35:111–118PubMedGoogle Scholar
  12. Kawabe H, Tanaka T, Mitshuhashi S (1978) Streptomycin and spectinomycin resistance mediated by plasmids. Antimicrobial Agents Chemother 13:1031–1035Google Scholar
  13. Khan SA, Novick RP (1983) Complete nucleotide sequence of pT181 a tetracycline-resistance plasmid from Staphylococcus aureus. Plasmid 10:251–259PubMedCrossRefGoogle Scholar
  14. Lehrach H, Diamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751PubMedCrossRefGoogle Scholar
  15. Maxam A, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65:499–560PubMedCrossRefGoogle Scholar
  16. McLaughlin JR, Murray CL, Rabinowitz JC (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus β-lactamase gene. J Biol Chem 256:11283–11291PubMedGoogle Scholar
  17. Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:10–89Google Scholar
  18. Murphy E (1983) Inhibition of Tn554 transposition: deletion analysis. Plasmid 10:260–269PubMedCrossRefGoogle Scholar
  19. Murphy E (1985) Nucleotide sequence of ermA, a macrolide-lineosamide-streptogramin B determinant in Staphylococcus avreus. J Bacteriol 162:633–640PubMedGoogle Scholar
  20. Murphy E, Löfdahl S (1984) Transposition of Tn554 does not generate a target duplication. Nature 307:292–294PubMedCrossRefGoogle Scholar
  21. Ozanne B, Benveniste R, Tipper D, Davies J (1969) Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors. J Bacteriol 100:1144–1146PubMedGoogle Scholar
  22. Phillips S, Novick RP (1979) A site-specific repressor-controlled transposon in Staphylococcus aureus. Nature 278:476–478PubMedCrossRefGoogle Scholar
  23. Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. J Mol Biol 113:237–251PubMedCrossRefGoogle Scholar
  24. Rosenberg M, Court D (1979) Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353PubMedCrossRefGoogle Scholar
  25. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467PubMedCrossRefGoogle Scholar
  26. Shine J, Dalgarno L (1974) The 3′ terminal sequence of Escherichia coli 16S ribosomal RNA: Complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71:1342–1346PubMedCrossRefGoogle Scholar
  27. Suzuki I, Takahashi N, Shirato S, Kawabe H, Mitsuhashi S (1975) Adenylylation of streptomycin by Staphylococcus aureus: A new streptomycin adenylyltransferase. In: Mitsuhashi S, Hashimoto H (eds) Microbial Drug Resistance. University of Tokyo Press, pp 463–474Google Scholar
  28. Thompson CJ, Gray GS (1983) Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. Proc Natl Acad Sci USA 80:5190–5194PubMedCrossRefGoogle Scholar
  29. Tu C, Cohen SN (1980) 3′ end labeling of DNA with α-32P-cordycepin-5′-triphosphate. Gene 10:177–183PubMedCrossRefGoogle Scholar
  30. Walker JB, Skorvaga M (1973) Phosphorylation of streptomycin and dihydrostreptomycin by Streptomyces. J Biol Chem 248:2435–2440PubMedGoogle Scholar
  31. Yagasawa M, Davies J (1979) Possible homology between genes for two aminoglycoside nucleotidyltransferases, AAD(3″) and ANT(2″): An evolutionary relationship? J Antibiot (Tokyo) 32:250–253Google Scholar
  32. Yamada D, Tipper D, Davies J (1968) Enzymatic inactivation of streptomycin by R-factor resistant Escherichia coli. Nature 219:288–291PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Ellen Murphy
    • 1
  1. 1.Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.New YorkUSA

Personalised recommendations