Molecular and General Genetics MGG

, Volume 200, Issue 1, pp 33–39 | Cite as

Nucleotide sequence of a spectinomycin adenyltransferase AAD(9) determinant from Staphylococcus aureus and its relationship to AAD(3″) (9)

  • Ellen Murphy


The nucleotide sequence of the spc determinant of the Staphylococcus aureus transposon Tn554 has been determined. This gene encodes a spectinomycin adenyltransferase, AAD(9), that mediates resistance to spectinomycin but not to streptomycin. The sequence predicts a 260 amino acid protein of molecular weight 28,943. A spectinomycin-sensitive mutant (spc-1) contains a G→A transition resulting in substitution of threonine (ACA) for alanine (GCA) at residue 165. The predicted amino acid sequence is 36% homologous to that of a widely distributed, gramnegative streptomycin/spectinomycin adenyltransferase, AAD(3″) (9), specified by the aadA determinant (Holingshead and Vapnek 1985).


Spectinomycin Protected Fragment Ribosome Binding Site Sequence LysTyr Benzyloxymethyl 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Ellen Murphy
    • 1
  1. 1.Department of Plasmid BiologyThe Public Health Research Institute of the City of New York, Inc.New YorkUSA

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