A fungal isolate, Arthrographis sp. strain F4, when grown in shake-flask culture, produced cellulolytic and xylanolytic enzymes optimally at 30°C with an initial pH of 5.0 to 6.0. Coarsely-ground filter paper was the most suitable carbon substrate for production of the enzymes. Inorganic nitrogen sources gave higher activities of the enzymes than organic nitrogen sources: NH4NO3 and yeast extract was the most effective combination. Significant stimulation (P<0.05) of enzyme production was achieved with 0.1% (v/v) Tween 80.
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B.C. Okeke was and S.K.C. Obi is with the Department of Microbiology, University of Nigeria, Nsukka, Nigeria. B.C. Okeke is now with the Department of Bioscience and Biotechnology, Royal College Building, University of Strathclyde, Glasgow G1 1XW, UK
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Okeke, B.C., Obi, S.K.C. Production of cellulolytic and xylanolytic enzymes by an Arthrographis species. World J Microbiol Biotechnol 9, 345–349 (1993). https://doi.org/10.1007/BF00383077
- Arthrographis sp.
- cellulolytic enzymes
- culture conditions
- xylanolytic enzymes