A quantitative assessment of the antimicrobial activity of garlic (Allium sativum)

  • L. P. Rees
  • S. F. Minney
  • N. T. Plummer
  • J. H. Slater
  • D. A. Skyrme
Research Paper


An aqueous extract of freeze-dried garlic (Allium sativum), when incorporated into growth media, inhibited many representative bacteria, yeasts, fungi and a virus. All microorganisms tested were susceptible to garlic. Quantitative assessment of the minimum inhibitory concentrations for bacteria and yeasts showed values ranging from 0.8 to 40.0 mg garlic ml-1. Fungal radial colony growth was inhibited by at least 25% at concentrations as low as 2.0 mg garlic ml-1. The 50% endpoint neutralization titre for rotavirus was 2.4 to 2.8 μg ml-1. Lactic acid bacteria were the least sensitive microorganisms to the inhibitory effects of garlic. In mixed culture studies of Lactobacillus acidophilus and Escherichia coli, garlic prevented the establishment of E. coli, although the final outcome of competition was not affected.

Key words

Anti-microbial activity competition garlic MIC values mixed culture 


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  1. Adetumbi, M., Javor, G.T. & Lau, B.H.S. 1986 Allium sativum (garlic) inhibits lipid synthesis in Candida albicans. Antimicrobial Agents and Chemotherapy 30, 499–501.PubMedGoogle Scholar
  2. Amer, M., Taha, M. & Tosson, Z. 1980 The effect of aqueous garlic extract on the growth of dermatophytes. International Journal of Dermatology 19, 285–287.PubMedGoogle Scholar
  3. Brydan, A.S., Davies, H.A., Thouless, M.E. & Flewett, T.H. 1977 Diagnosis of rotavirus infection by cell culture. Journal of Medical Microbiology 10, 121–125.PubMedGoogle Scholar
  4. Eagle, H. & Musselman, A.D. 1948 The rate of bactericidal action of penicillin in vitro as a function of its concentrations, and its paradoxically reduced activity at high concentrations against certain organisms. Journal of Experimental Medicine 88, 99–131.CrossRefGoogle Scholar
  5. Fromtling, R. & Bulmer, G.S. 1978 In vitro effect of aqueous extract of garlic (Allium sativum) on the growth and viability of Cryptococcus neoformis. Mycologia 70, 397–405.PubMedGoogle Scholar
  6. Ghannoum, M.A. 1988 Studies of the antimicrobial mode of action of Allium sativum (garlic). Journal of General Microbiology 134, 2917–2924.PubMedGoogle Scholar
  7. Huddleson, I.F., DuFrain, J., Barrows, K.C. & Giefel, M. 1944 Antibacterial substances in plants. Journal of the American Veterinary Medicine Association 105, 394–412.Google Scholar
  8. Iberl, B., Winkler, G., Muller, B. & Knobloch, K. 1990 Quantitative determination of allicin and Alliin from garlic by HPLC. Planta Medica 56, 320–326.Google Scholar
  9. Jezpwa, L., Rafinski, T.J. & Wrocinski, T. 1966 Investigations on the antibiotic activity of Allium sativum. Herba Polanica 12, 3–7.Google Scholar
  10. Miles, A.A. & Misra, S.S. 1938 The estimation of the bacterial power of the blood. Journal of Hygiene 36, 732–749.Google Scholar
  11. Nagai, K. 1973 Experimental studies on the preventative effect of garlic extract against infection with influenza virus. Japanese Journal of Infectious Diseases 47, 321–325.Google Scholar
  12. Sharma, V.D., Sethi, M.S., Kumar, A. & Rarotra, J.R. 1977 Antibacterial property of Allium sativum Lill: in vitro and in vivo studies. Indian Journal of Experimental Biology 15, 466–468.PubMedGoogle Scholar
  13. Tsai, Y., Cole, L., Davis, L., Lockwood, S., Simmons, V. & Wild, W. 1985 Antiviral properties of garlic: In vitro effects on Influenza B, Herpes Simplex and Coxsackie viruses. Planta Medica 5, 460–461.PubMedGoogle Scholar
  14. Tynecka, A. & Gos, Z. 1975 The fungistatic activity of garlic (Allium sativum) in vitro. Annales Universitatis Mariae Curie-Sklodowska 30, 5–13.Google Scholar
  15. Wills, E.D. 1956 Enzyme inhibition by allicin, the active principle of garlic. Biochemical Journal 63, 514–520.PubMedGoogle Scholar

Copyright information

© Rapid Communications of Oxford Ltd 1993

Authors and Affiliations

  • L. P. Rees
  • S. F. Minney
  • N. T. Plummer
  • J. H. Slater
  • D. A. Skyrme

There are no affiliations available

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