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Molecular and General Genetics MGG

, Volume 169, Issue 3, pp 229–235 | Cite as

X-Ray sensitivity of Escherichia coli lysogenic for bacteriophage P2

  • Daniela Ghisotti
  • Sandro Zangrossi
  • Gianpiero Sironi
Article

Summary

Strains of Escherichia coli C or K lysogenic for the non-inducible phage P2 show a lower survival following X-ray irradiation as compared to nonlysogenic strains. This difference in X-ray sensitivity is not accompanied by a significant difference in X-ray induced mutability. The capacity of X-irradiated P2 lysogens to multiply any of a number of unirradiated infecting phages is severely impaired. These effects of X-ray treatment can be most simply explained as a consequence of the fact that protein and RNA syntheses are strongly inhibited in P2 lysogens after X-irradiation. All the above events specifically occurring in X-rayed P2 lysogens are dependent on the P2 gene old.

Keywords

Escherichia Coli Lower Survival Induce Mutability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson, T.F.: The growth of T2 virus on ultraviolet-killed host cells. J. Bacteriol. 56, 403–410 (1948)Google Scholar
  2. Appleyard, R.K.: Segregation of new lysogenic types during growth of a double lysogenic strain derived from Escherichia coli. Genetics 39, 440–452 (1954)Google Scholar
  3. Barbour, S.D., Clark, A.J.: Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB + and recC + genes. Proc. Natl. Acad. Sci. U.S.A. 65, 955–961 (1970)Google Scholar
  4. Barksdale, L., Arden, S.B.: Persisting bacteriophage infections, lysogeny and phage conversions. Annu. Rev. Microbiol. 28, 265–300 (1974)Google Scholar
  5. Bertani, G.: Deletions in bacteriophage P2. Circularity of the genetic map and its orientation relative to the DNA denaturation map. Mol. Gen. Genet. 136, 107–137 (1975)Google Scholar
  6. Bertani, L.E.: Host-dependent induction of phage mutants and lysogenization. Virology 12, 553–569 (1960)Google Scholar
  7. Bertani, L.E.: Lysogenic conversion by bacteriophage P2 resulting in an increased sensitivity of Escherichia coli to 5-fluorodeoxyuridine. Biochim. Biophys. Acta 87, 631–640 (1964)Google Scholar
  8. Bertani, L. E.: Abortive induction of bacteriophage P2. Virology 36, 87–103 (1968)Google Scholar
  9. Bertani, L. E.: Characterization of clear mutants belonging to the Z gene of bacteriophage P2. Virology 71, 85–96 (1976)Google Scholar
  10. Bertani, L.E., Bertani, G.: Genetics of P2 and related phages. Adv. Genet. 16, 199–237 (1971)Google Scholar
  11. Bertani, L.E., Levy, J.A.: Conversion of lysogenic Escherichia coli by non-multiplying, superinfecting bacteriophage P2. Virology 22, 634–640 (1964)Google Scholar
  12. Brégégère, F.: Bacteriophage P2-λ interference: inhibition of protein synthesis involves transfer RNA inactivation. J. Mol. Biol. 90, 459–467 (1974)Google Scholar
  13. Brégégère, F.: Bacteriophage P2-λ interference. II Effects on the host under the control of lambda genes O and P. J. Mol. Biol. 104, 411–420 (1976)Google Scholar
  14. Brégégère, F.: Bacteriophage P2-λ interference. III Essential role of an early step in the initiation of lambda replication. J. Mol. Biol. 122, 113–125 (1978)Google Scholar
  15. Chattoraj, D.K., Inman, R.B.: Position of two deletion mutations on the physical map of bacteriophage P2. J. Mol. Biol. 66, 423–434 (1972)Google Scholar
  16. Chattoraj, D.K., Inman, R.B.: Electron microscope heteroduplex mapping of P2 Hy dis bacteriophage DNA. Virology 55, 174–182 (1973)Google Scholar
  17. Choe, B.K.: Integration defective mutants of bacteriophage P2. Mol. Gen. Genet. 105, 275–284 (1969)Google Scholar
  18. Cohen, D.: A variant of phage P2 originating in Escherichia coli, strain B. Virology 7, 112–126 (1959)Google Scholar
  19. Howard-Flanders, P., Theriot, L.: Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53, 1137–1150 (1966)PubMedGoogle Scholar
  20. Labaw, L.W., Mosley, V.M., Wyckoff, R.W.: Development of bacteriophage in X-ray inactivated bacteria. J. Bacteriol. 65, 330–336 (1953)Google Scholar
  21. Lindahl, G.: Multiple recombination mechanisms in bacteriophage P2. Virology 39, 861–866 (1969)Google Scholar
  22. Lindahl, G.: Bacteriophage P2: replication of the chromosome requires a protein which acts only on the genome that coded for it. Virology 42, 522–533 (1970)Google Scholar
  23. Lindahl, G.: Characterization of conditional lethal mutants of bacteriophage P2. Mol. Gen. Genet. 128, 249–260 (1974)Google Scholar
  24. Lindahl, G., Sironi, G., Bialy, H., Calendar, R.: Bacteriophage lambda; Abortive infection of bacteria lysogenic for phage P2. Proc. Natl. Acad. Sci. U.S.A. 66, 587–594 (1970)Google Scholar
  25. Lindahl, G., Sunshine, M.: Excision-deficient mutants of bacteriophage P2. Virology 49, 180–187 (1972)Google Scholar
  26. Sakaki, Y., Karu, A.E., Linn, S., Echols, H.: Purification and properties of the γ-protein specified by bacteriophage λ: An inhibitor of the host recBC recombination enzyme. Proc. Natl. Acad. Sci. U.S.A. 70, 2215–2219 (1973)Google Scholar
  27. Sasaki, I., Bertani, G.: Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J. Gen. Microbiol. 40, 365–376 (1965)Google Scholar
  28. Sironi, G.: Mutants of Escherichia coli unable to be lysogenized by the temperate bacteriophage P2. Virology 37, 163–176 (1969)Google Scholar
  29. Sironi, G., Bialy, H., Lozeron, H.A., Calendar, R.: Bacteriophage P2: interaction with phage lambda and with recombination-deficient bacteria. Virology 46, 387–396 (1971)Google Scholar
  30. Sussman, R., Jacob, F.: Sur un système de répression thermosensible chez le bacteriophage λ d'Escherichia coli. C.R. Acad. Sci. 254, 1517–1519 (1962)Google Scholar
  31. Suzuki, H., Nagai, K., Yamaki, H., Tanaka, N., Umezawa, H.: On the mechanism of action of bleomycin: scission of DNA strands in vitro and in vivo. J. Antibiot. (Tokyo) 22, 446–448 (1969)Google Scholar
  32. Unger, R.C., Clark, A.J.: Interaction of the recombination pathways of bacteriophage λ and its host Escherichia coli K12: effects on exonuclease V activity. J. Mol. Biol. 70, 539–548 (1972)Google Scholar
  33. Thomas, R., Bertani, L.E.: On the control of the replication of temperate bacteriophages superinfecting immune hosts. Virology 24, 241–253 (1964)Google Scholar
  34. White, H.L., White, J.R.: Interaction of streptonigrin with DNA in vitro. Biochim. Biophys. Acta 123, 648–650 (1966)Google Scholar
  35. Wiman, M., Bertani, G., Kelly, B., Sasaki, I.: Genetic map of Escherichia coli strain C. Mol. Gen. Genet. 107, 1–31 (1970)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Daniela Ghisotti
    • 1
  • Sandro Zangrossi
    • 1
  • Gianpiero Sironi
    • 1
  1. 1.Instituto di GeneticaUniversità di MilanoMilanoItaly

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