Archives of Microbiology

, Volume 163, Issue 2, pp 138–142

Proline betaine is a highly effective osmoprotectant for Staphylococcus aureus

  • Ukti S. Amin
  • Timothy D. Lash
  • Brian J. Wilkinson
Original Paper


Proline betaine is an osmoprotectant that is at least as effective as glycine betaine, and more effective than L-proline, for various strains of Staphylococcus aureus, and Staphylococcus epidermidis and Staphylococcus saprophyticus. 13C NMR studies revealed that proline betaine accumulated to high levels in osmotically stressed S. aureus, but was also detected in organisms grown in its presence in the absence of osmotic stress. Competition experiments indicated that proline betaine was taken up by the proline transport systems of S. aureus, but not by the high affinity glycine betaine transport system.

Key words

Staphylococcus aureus Osmoregulation Proline betaine Nuclear magnetic resonance spectroscopy Coagulase-negative staphylococci 




Yeast extract



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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Ukti S. Amin
    • 1
    • 2
  • Timothy D. Lash
    • 2
  • Brian J. Wilkinson
    • 1
    • 2
  1. 1.Microbiology Group, Department of, Biological SciencesIllinois State UniversityNormalUSA
  2. 2.Department of ChemistryIllinois State UniversityNormalUSA

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