Biological monitoring of 2,4-dichlorophenoxyacetic acid-exposed workers in agriculture and forestry

  • Dietmar Knopp
  • Steffi Glass

DOI: 10.1007/BF00381583

Cite this article as:
Knopp, D. & Glass, S. Int. Arch Occup Environ Heath (1991) 63: 329. doi:10.1007/BF00381583


This is a report on the application of a radioimmunossay (RIA) for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) in biological monitoring of occupationally exposed sprayers. Urinalysis was conducted on two workers involved in spraying 2,4-D sodium salt solution with car mounted ground rigs in agriculture and on the pilot and the mixer-loader of a helicopter crew applying 2,4-D dimethylamine salt for brush control in forestry. All sprayers showed detectable quantitities of 2,4-D in morning urine samples voided over 4 or 6 days in the post-spraying period. The highest 2,4-D urinary concentrations of about 2.5 ppm could be measured in a car driver on the 3 day after exposure. The 2,4-D level in urine was much lower in forestry workers, with 0.365 ppm for the mixer-loader and 0.052 ppm for the pilot on the 1 day after spraying. Urinary 2,4-D concentrations in the agricultural study were adjusted for endogenous creatinine and, when normalized for body weight, resulted in a total 2,4-D uptake of about 5.7 or 84.9 μ/kg body wt. for a single spraying operation. Thus, the calculated absorbed dose was less than the NOAEL (no observed adverse effect level) of 10 mg/kg body weight per day by a large margin of safety. Further, the calculated amounts excreted seemed to be sufficiently reliable to be used for assessing the risk of human exposure to 2,4-D. However, more studies are desirable for confirmation of the association of urinary 2,4-D and creatinine excretion. Clearance occurs with a 12-h to 22-h half-life.

Key words

2,4-D Immunoassay Biological monitoring Exposure Human 

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Dietmar Knopp
    • 1
  • Steffi Glass
    • 2
  1. 1.Institute for Water, Soil and Air Hygiene of the Federal Health OfficeResearch Department Bad ElsterBad ElsterGermany
  2. 2.Municipal Out-Patient DepartmentBad ElsterGermany

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