Biological monitoring of workers exposed to styrene and acetone

  • E. De Rosa
  • M. Cellini
  • G. Sessa
  • C. Saletti
  • G. Rausa
  • G. Marcuzzo
  • G. B. Bartolucci
Article

Summary

Twenty-two workers exposed to styrene and acetone in two fiberglass industries were monitored on Monday and Thursday for 8 hours using passive dosimeters. Urine samples were collected at the end of the workshift and before the start of the work on the next morning (Tuesday and Friday). The charcoal disks of the passive dosimeters were analysed by gas-chromatography. Mandelic acid (MA) and phenylglyoxylic acid (PGA) were measured using a HPLC method; values were expressed in mg/g of creatinine. The 8-h TWA exposure values for styrene and acetone ranged respectively from 22 to 522 mg/m 3 and 40-1581 mg/m on Monday; 25–423 mg/m3 and 55–579 mg/m3 on Thursday. Styrene TWA exposure values significantly correlate with the sum of metabolites at the end of workday (r = 0.70 on Monday and r = 0.95 on Thursday) and also at the next morning (r = 0.86 on Tuesday and r = 0.85 on Friday). A styrene exposure level of 213 mg/m3 (ACGIH-TLV) was associated with an excretion of metabolites (MA+PGA) higher on Thursday (803 mg/g Great) than on Monday (570 mg/g creat). The same result was found on Friday (459 mg/g creat) compared with Tuesday (305 mg/g creat). Moreover our data show that the simultaneous exposure to acetone does not modify the excretion of MA. In conclusion the TLV of styrene is associated with different values of metabolites at the beginning and at the end of the work-week.

Key words

Acetone Biological monitoring Fiberglass Industry Mandelic and phenylglyoxylic acids Styrene 
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References

  1. 1.
    ACGIH 1991–92 (1991) Threshold limit values for chemical substances and physical agents and biological exposure indices. Cincinnati, OHGoogle Scholar
  2. 2.
    Bartolucci GB, De Rosa E, God GP, Chiesura Corona P, Perbellini L, Brugnone F (1986) Biomonitoring of occupational exposure to styrene. Appl Ind Hyg 3:125–131Google Scholar
  3. 3.
    Berode M, Droz PO, Boillat MA, Guillemin MP (1986) Effect of alcohol on the kinetics of styrene and its metabolites in volunteers and in workers. Appl Ind Hyg 1:25–28Google Scholar
  4. 4.
    De Rosa E, Bartolucci GB, Perbellini L, Brugnone F, Rausa G (1988) Environmental and biological monitoring of exposure to toluene, styrene, and n-hexane. Appl Ind Hyg 3:332–337Google Scholar
  5. 5.
    Droz PO (1985) The use of simulation models for setting BEIs for organic solvents. Ann Am Conf Ind Hyg 12:339–350Google Scholar
  6. 6.
    Engström K, Härkönen H, Kalliokoski P, Rantanen J (1976) Urinary mandelic acid concentration after occupational exposure to styrene and its use as a biological exposure test. Scand J Work Environ Health 2:21–26PubMedGoogle Scholar
  7. 7.
    Ferioli A, Bolsieri L, Apostoli P, Buizza P (1989) Interferenza dell'acetone sul metabolismo dello stirene. Dati preliminari osservati in lavoratori del settore vetroresina. Lavoro e Medicina 1:113–115Google Scholar
  8. 8.
    Fields RL, Horstman SW (1979) Biomonitoring of industrial styrene exposure. Am Ind Hyg Assoc J 40:451–459PubMedGoogle Scholar
  9. 9.
    Guillemin MP, Berode M (1988) Biological monitoring of styrene: a review. Am Ind Hyg Assoc J 49:497–505PubMedGoogle Scholar
  10. 10.
    Ikeda M, Hirayama T (1978) Possible metabolic interaction of styrene with organic solvents. Scand J Work Environ Health 4 (Suppl. 2): 41–46PubMedGoogle Scholar
  11. 11.
    Ikeda M, Ohtsuji H, Imamura T (1972) In vivo suppression of benzene and styrene oxidation by co-administered toluene in rats and effects of phenobarbital. Xenobiotica 2:101–106PubMedGoogle Scholar
  12. 12.
    NIOSH (1977) Manual of analytical methods. 2nd Ed. Vol II. DHEW Pub. N° 77-157-B Cincinnati, OHGoogle Scholar
  13. 13.
    Poggi G, Giusiani M, Palagi U, Paggiaro PL, Loi AM, Dazzi F, Siclari C, Baschieri L (1982) High-performance liquid chromatography for the quantitative determination of the urinary metabolites of toluene, xilene and styrene. Int Arch Occup Environ Health 50:25–31PubMedGoogle Scholar
  14. 14.
    Vesterberg O, Sollenberg J, Wzangskog K (1985) Evaluation of determinations made in urine samples. Adjustments of mandelic acid concentration using creatinine and density. Ann Am Conf Ind Hyg 12:301–305Google Scholar
  15. 15.
    Wigaeus E, Löf A, Byfält-Nordquist M (1984) Uptake, distribution, metabolism and elimination of styrene in man. A comparison between single exposure and co-exposure to acetone. Br J Ind Med 41:539–546PubMedGoogle Scholar
  16. 16.
    Wilson HK, Robertson SM, Waldron HA, Gompertz D (1983) Effect of alcohol on the kinetics of mandelic acid excretion in volunteers exposed to styrene vapour. Br J Ind Med 40:75–80PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • E. De Rosa
    • 1
  • M. Cellini
    • 2
  • G. Sessa
    • 2
  • C. Saletti
    • 3
  • G. Rausa
    • 3
  • G. Marcuzzo
    • 4
  • G. B. Bartolucci
    • 4
  1. 1.Institute of Hygiene and Preventive Medicine, University of FerraraFerraraItaly
  2. 2.Occupational Health Inspectorate (S.M.P.I.L.)FerraraItaly
  3. 3.Institute of Hygiene and Preventive Medicine, University of FerraraFerraraItaly
  4. 4.Institute of Occupational Medicine, University of PadovaPadovaItaly

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