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Effects of abscisic acid, gibberellic acid and fusicoccin on the transmembrane potential during the early phases of germination in radish (Raphanus sativus L.) seeds

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Abstract

During germination, the transmembrane electric potential (PD) of cortical cells of the embryonal axis of radish seeds (Raphanus sativus L.) rises from-120 mV initially to a maximum of-150 mV after 5 h incubation, then falls again to stable values of around-120 mV. Treatments inhibiting germination block the transitory PD increase. Administration of uncoupling agents or low temperatures, during the process of germination, produces a marked fall of the PD transitory increase. Abscisic Acid has a parallel inhibitory effect on PD and germination, while fusicoccin produces a rise in both; administration of abscisic acid with fusicoccin inhibits germination, while the PD remains at the high levels given by fusicoccin. These results are discussed in relation to ion exchange at membrane level.

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Abbreviations

ABA:

abscisic acid

FC:

fusicoccin

GA3 :

gibberellic acid

PD:

electric potential difference (between the vacuole and the external medium)

CH:

cycloheximide

DNP:

dinitrophenol

FCCP:

(p-trifluormethoxy)-carbonylcyanide-phenylhydrazone

DCCD:

N,N′-dicyclohexylcarbodiimide

References

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Ballarin-Denti, A., Cocucci, M. Effects of abscisic acid, gibberellic acid and fusicoccin on the transmembrane potential during the early phases of germination in radish (Raphanus sativus L.) seeds. Planta 146, 19–23 (1979). https://doi.org/10.1007/BF00381250

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Key words

  • Abscisic acid
  • Fusicoccin
  • Germination (seeds)
  • Gibberellin
  • Raphanus
  • Transmembrane potential