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Planta

, Volume 146, Issue 4, pp 393–398 | Cite as

Transport of proteins from cytoplasm into plastids in chloramphenicol-treated bean leaf discs

Autoradiographic evidence
  • Kazimierz Strzałka
  • Maria Kwiatkowska
Article

Abstract

Leaf discs from etiolated bean plants were found to incorporate [3H]lysine into 80 S ribosomesynthesized proteins in the presence of chloramphenicol (100 mg l−1) when exposed to light. After a 7 min pulse of [3H]lysine, the discs were transferred to the same medium but with nonradioactive lysine, and postincubation was carried out for 24 h. The number of silver grains over the plastids, after the first period of a lag phase, indicates a large increase between 12 and 24 h of postincubation. Simultaneously, the labeling of the cytoplasm becomes reduced during that period. The results show that during inhibition of the protein formation within plastids, the synthesis of plastid-destined proteins in cytoplasm, as well as their transport into plastids, can still proceed.

Key words

Autoradiography Chloramphenicol Phaseolus Plastids Protein synthesis Transport 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Kazimierz Strzałka
    • 1
  • Maria Kwiatkowska
    • 2
  1. 1.Department of Plant Biochemistry, Institute of Molecular BiologyJagellonian UniversityKrakôwPoland
  2. 2.Department of Plant Cytology and Cytochemistry, Institute of Physiology and CytologyUniversity of ŁódźŁodzPoland

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