, Volume 146, Issue 4, pp 393–398 | Cite as

Transport of proteins from cytoplasm into plastids in chloramphenicol-treated bean leaf discs

Autoradiographic evidence
  • Kazimierz Strzałka
  • Maria Kwiatkowska


Leaf discs from etiolated bean plants were found to incorporate [3H]lysine into 80 S ribosomesynthesized proteins in the presence of chloramphenicol (100 mg l−1) when exposed to light. After a 7 min pulse of [3H]lysine, the discs were transferred to the same medium but with nonradioactive lysine, and postincubation was carried out for 24 h. The number of silver grains over the plastids, after the first period of a lag phase, indicates a large increase between 12 and 24 h of postincubation. Simultaneously, the labeling of the cytoplasm becomes reduced during that period. The results show that during inhibition of the protein formation within plastids, the synthesis of plastid-destined proteins in cytoplasm, as well as their transport into plastids, can still proceed.

Key words

Autoradiography Chloramphenicol Phaseolus Plastids Protein synthesis Transport 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Blobel, G., Dobberstein, B.: Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains of membrane bound ribosomes of murine myeloma. J. Cell. Biol. 67, 835–851 (1975)Google Scholar
  2. Campbell, P.N., Blobel, G.: The role of organelles in the chemical modification of the primary translation products of secretory proteins. FEBS Lett. 72, 215–226 (1976)Google Scholar
  3. Cobb, A.H., Wellburn, A.R.: Developmental changes in the levels of SDS-extractable polypeptides during plastid morphogenesis. Planta 114, 131–142 (1973)Google Scholar
  4. Cobb, A.H., Wellburn, A.R.: Changes in plastid envelope polypeptides during chloroplast development. Planta 121, 273–282 (1974)Google Scholar
  5. Cobb, A.H., Wellburn, A.R.: Polypeptide binding to plastid envelopes during chloroplast development. Planta 129, 127–132 (1976)Google Scholar
  6. Cockburn, B.J., Wellburn, A.R.: Changes in the envelope permeability of developing chloroplasts. J. Exp. Bot. 25, 36–49 (1974)Google Scholar
  7. Ellis, R.J.: Protein and nucleic acid synthesis by chloroplasts. In: Topics in photosynthesis, vol. I: The intact chloroplast, pp. 335–364, Barber, J., ed. Amsterdam: Elsevier 1976Google Scholar
  8. Feierabend, J., Mikus, M.: Occurence of a high temperature sensitivity of chloroplast ribosome formation in several higher plants. Plant Physiol. 59, 863–867 (1977)Google Scholar
  9. Feierabend, J., Schrader-Reichhardt, U.: Biochemical differnetiation of plastids and other organelles in rye leaves with a high-temperature-induced deficiency of plastid ribosomes. Planta 129, 133–145 (1976)Google Scholar
  10. Feierabend J., Wildner, G.: Formation of the small subunit in the absence of the large subunit of ribulose-1,5-bisphosphate carboxylase in 70 S ribosome deficient rye leaves. Arch. Biochem. Biophys. 186, 283–291 (1978)Google Scholar
  11. Gooding, L.R., Roy, H., Jagendorf, A.T.: Immunological identification of nascent subunits of wheat ribulose diphosphate carboxylase on ribosomes of both chloroplast and cytoplasmic origin. Arch. Biochem. Biophys. 159, 324–335 (1973)Google Scholar
  12. Hampp, R., Schmidt, H.W.: Changes in envelope permeability during chloroplast development. Planta 129, 69–74 (1976)Google Scholar
  13. Hampp, R., Wellburn, A.R.: Early changes in envelope permeability of developing chloroplasts J. Exp. Bot. 27, 778–784 (1976)Google Scholar
  14. Highfield, P.E., Ellis, R.J.: Synthesis and transport of the small subunit of chloroplast ribulose bisphosphate carboxylase. Nature 271, 420–424 (1978)Google Scholar
  15. Hoober, K.J., Stegeman, W.J.: Control of the synthesis of a major polypeptide of chloroplast membranes in Chlamydomonas reinhardii. J Cell. Biol. 56, 1–12 (1973)Google Scholar
  16. Kirk, J.T.O., Tilney-Bassett, R.A.E.: The plastids. London, San Francisco: Freeman 1967Google Scholar
  17. Laulhere, J.P., Dorne, A.M.: Are cytoplasmic ribosomes in chloroplast preparations functionally attached to the chloroplast membrane? Plant Sci. Lett 8, 251–256 (1977)Google Scholar
  18. Schäfers, H.A., Feierabend, J.: Ultrastructural differentiation of plastids and other organelles in rye leaves with high-temperature induced deficiency of plastid ribosomes. Cytobiologie 14, 75–90 (1976)Google Scholar
  19. Strzałka, K., Majewska, G., Mędrela, E.: Effects of chloamphenicol and cycloheximide on the relative contents of chlorophyll and protein in various subchloroplast fractions. Physiol. Plant. (in press)Google Scholar
  20. Walles, B.: Plastid inheritance and mutations. In: Structure and function of chloroplasts, pp. 51–88. Gibbs, M., ed. Berlin, Heidelberg, New York: Springer 1971Google Scholar
  21. Woodcock, C.L.F., Bogorad, L.: Nucleic acids and information processing in chloroplasts. In: Structure and function of chloroplasts, pp. 89–128. Gibbs, M., ed. Berlin, Heidelberg, New York: Springer 1971Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Kazimierz Strzałka
    • 1
  • Maria Kwiatkowska
    • 2
  1. 1.Department of Plant Biochemistry, Institute of Molecular BiologyJagellonian UniversityKrakôwPoland
  2. 2.Department of Plant Cytology and Cytochemistry, Institute of Physiology and CytologyUniversity of ŁódźŁodzPoland

Personalised recommendations