In vitro biosynthesis of the C-glycosidic bond in aloin
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Biosynthetic studies with cell-free extracts from Aloe arborescens Mill. demonstrate the transfer of the glucose moiety from UDP-glucose to aloe emodin anthrone, forming the C-glycosidic linkage in the anthracene derivative aloin. The pH-dependence and the specificity of UDP-glucose and aloe emodin anthrone for the biosynthesis of the C-glycosidic bond in aloin are shown.
Key wordsAloe Aloin Glucosylation (C-) Glycosides (C-, biosynthesis)
aloe emodin anthrone (1,8-dihydroxy-3-(hydroxymethyl)-9(10 H)-anthracenone)
acetyl coenzyme A
high performance liquid chromatography
thin layer chromatography
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