, Volume 127, Issue 3, pp 285–299 | Cite as

Relative thermostability of the chloroplast envelope

  • G. H. Krause
  • K. A. Santarius


Intact isolated chloroplasts from leaves of Spinacia oleracea L. were subjected to heat treatment. After heating, the integrity of the chloroplast envelopes and the activities of various light-dependent chloroplast reactions were tested. The integrity of the chloroplast envelopes, as judged from rates of ferricyanide reduction, enzyme compartmentation and visual appearance of the chloroplasts in the light microscope with phase optics, was affected much less by heat stress than the photochemical reactions of thylakoids. This indicates a comparatively high thermostability of the chloroplast envelope membranes. It is also evidence of a differential thermostability of different biomembranes. Photophosphorylation was highly susceptible to thermal stress. Heat treatment that partly inactivated phosphorylation stimulated light-dependent quenching of 9-aminoacridine fluorescence, which served as an indicator of proton transfer from stroma to thylakoids in intact chloroplasts. Drastic changes in the characteristics of chlorophyll a fluorescence emission caused by heating were probably due to structural alterations of the thylakoid system.


Thermal Stress Heat Stress Proton Transfer Ferricyanide Phase Optic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • G. H. Krause
    • 1
  • K. A. Santarius
    • 1
  1. 1.Botanisches Institute der Universität DüsseldorfDüsseldorf 1Germany

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