Zeitschrift für Parasitenkunde

, Volume 53, Issue 2, pp 133–141 | Cite as

The action of metabolic inhibitors on microgametogenesis in Plasmodium yoelii nigeriensis

  • P. J. Toyé
  • R. E. Sinden
  • Elizabeth U. Canning


Mature gametocytes of Plasmodium yoelii nigeriensis were incubated in vitro with a variety of antibiotics whose activities were directed against DNA, RNA, protein and microtubule assembly. Following incubation for periods between 5 and 90 min, exflagellation was induced and the degree of inhibition produced by each drug was assayed microscopically.

The results obtained show that de novo synthesis of axonemes and proteins is essential to the events of microgametogenesis. The inhibitory effect of actinomycin-D suggests that de novo RNA synthesis is also required. However, the inactivity of 8-azaguanine, a drug also effective against m-RNA metabolism, casts some doubt on the requirement for m-RNA synthesis.

DNA synthesis is not required during the three successive mitotic divisions of the parasite genome during microgametogenesis. The mature microgametocyte therefore contains the 8c DNA required for microgamete production.


Plasmodium Metabolic Inhibitor Mitotic Division Microtubule Assembly Parasite Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • P. J. Toyé
    • 1
  • R. E. Sinden
    • 1
  • Elizabeth U. Canning
    • 1
  1. 1.Department of Zoology and Applied EntomologyImperial CollegeLondonUK

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